{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:06:23Z","timestamp":1784073983474,"version":"3.55.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mutations in the internal ribosome entry site (IRES) of hepatitis A virus (HAV) have been associated with enhanced\n            <jats:italic>in vitro<\/jats:italic>\n            replication and viral attenuation in animal models. To address the possible role of IRES variability in clinical presentation, IRES sequences were obtained from HAV isolates associated with benign (\n            <jats:italic>n<\/jats:italic>\n            = 8) or severe (\n            <jats:italic>n<\/jats:italic>\n            = 4) hepatitis. IRES activity was assessed using a bicistronic dual-luciferase expression system in adenocarcinoma (HeLa) and hepatoma (HuH7) cell lines. Activity was higher in HuH7 than in HeLa cells, except for an infrequently isolated genotype IIA strain. Though globally low, significant variation in IRES-dependent translation efficiency was observed between field isolates, reflecting the low but significant genetic variability of this region (94.2% \u00b1 0.5% nucleotide identity). No mutation was exclusive of benign or severe hepatitis, and variations in IRES activity were not associated with a clinical phenotype, indirectly supporting the preponderance of host factors in determining the clinical presentation.\n          <\/jats:p>","DOI":"10.1128\/jvi.02598-09","type":"journal-article","created":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T02:47:24Z","timestamp":1279162044000},"page":"10139-10147","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Nucleotide Variability and Translation Efficiency of the 5\u2032 Untranslated Region of Hepatitis A Virus: Update from Clinical Isolates Associated with Mild and Severe Hepatitis"],"prefix":"10.1128","volume":"84","author":[{"given":"Vincent","family":"Mackiewicz","sequence":"first","affiliation":[{"name":"AP-HP, Ho\u0302pital Paul Brousse Centre National de Re\u0301fe\u0301rence du Virus de l'He\u0301patite A, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Cammas","sequence":"additional","affiliation":[{"name":"INSERM U563, Institut Claudius Regaud, 20-24 rue du Pont Saint-Pierre, 31052 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Desbois","sequence":"additional","affiliation":[{"name":"AP-HP, Ho\u0302pital Paul Brousse Centre National de Re\u0301fe\u0301rence du Virus de l'He\u0301patite A, Villejuif, France"},{"name":"INSERM U785, 94804 Villejuif, France"},{"name":"Universite Paris-Sud, UMR-S 785, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Marchadier","sequence":"additional","affiliation":[{"name":"AP-HP, Ho\u0302pital Paul Brousse Centre National de Re\u0301fe\u0301rence du Virus de l'He\u0301patite A, Villejuif, 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