{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T21:41:58Z","timestamp":1792878118921,"version":"4.5.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,5,15]],"date-time":"2007-05-15T00:00:00Z","timestamp":1179187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CD8<jats:sup>+<\/jats:sup>T-cell responses control lymphocytic choriomeningitis virus (LCMV) infection in H-2<jats:sup>b<\/jats:sup>mice. Although antigen-specific responses against LCMV infection are well studied, we found that a significant fraction of the CD8<jats:sup>+<\/jats:sup>CD44<jats:sup>hi<\/jats:sup>T-cell response to LCMV in H-2<jats:sup>b<\/jats:sup>mice was not accounted for by known epitopes. We screened peptides predicted to bind major histocompatibility complex class I and overlapping 15-mer peptides spanning the complete LCMV proteome for gamma interferon (IFN-\u03b3) induction from CD8<jats:sup>+<\/jats:sup>T cells derived from LCMV-infected H-2<jats:sup>b<\/jats:sup>mice. We identified 19 novel epitopes. Together with the 9 previously known, these epitopes account for the total CD8<jats:sup>+<\/jats:sup>CD44<jats:sup>hi<\/jats:sup>response. Thus, bystander T-cell activation does not contribute appreciably to the CD8<jats:sup>+<\/jats:sup>CD44<jats:sup>hi<\/jats:sup>pool. Strikingly, 15 of the 19 new epitopes were derived from the viral L polymerase, which, until now, was not recognized as a target of the cellular response induced by LCMV infection. The L epitopes induced significant levels of in vivo cytotoxicity and conferred protection against LCMV challenge. Interestingly, protection from viral challenge was best correlated with the cytolytic potential of CD8<jats:sup>+<\/jats:sup>T cells, whereas IFN-\u03b3 production and peptide avidity appear to play a lesser role. Taken together, these findings illustrate that the LCMV-specific CD8<jats:sup>+<\/jats:sup>T-cell response is more complex than previously appreciated.<\/jats:p>","DOI":"10.1128\/jvi.02632-06","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T10:44:34Z","timestamp":1172745874000},"page":"4928-4940","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":107,"title":["The CD8<sup>+<\/sup>T-Cell Response to Lymphocytic Choriomeningitis Virus Involves the L Antigen: Uncovering New Tricks for an Old Virus"],"prefix":"10.1128","volume":"81","author":[{"given":"Maya F.","family":"Kotturi","sequence":"first","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjoern","family":"Peters","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Fernando","family":"Buendia-Laysa","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Sidney","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Oseroff","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Botten","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard","family":"Grey","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Buchmeier","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neurosciences Department, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.3.2081-2092.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00153-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.6.3925"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00085-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02063-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.4077"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80469-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1633254100"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.9.5226-5240.2003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02122426"},{"key":"e_1_3_2_12_2","first-page":"67","volume":"133","year":"1987","unstructured":"Francis, S. 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