{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:53:23Z","timestamp":1791312803690,"version":"4.3.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The potential threat of smallpox as a bioweapon has led to the production\nand stockpiling of smallpox vaccine in some countries. Human monkeypox,\na rare but important viral zoonosis endemic to central and western\nAfrica, has recently emerged in the United States. Thus, even though\nsmallpox has been eradicated, a vaccinia virus vaccine that can induce\nprotective immunity against smallpox and monkeypox is still invaluable.\nThe ability of the highly attenuated vaccinia virus vaccine strain\nLC16m8, with a mutation in the important immunogenic membrane protein\nB5R, to induce protective immunity against monkeypox in nonhuman\nprimates was evaluated in comparison with the parental Lister strain.\nMonkeys were immunized with LC16m8 or Lister and then infected\nintranasally or subcutaneously with monkeypox virus strain Liberia or\nZr-599, respectively. Immunized monkeys showed no symptoms of monkeypox\nin the intranasal-inoculation model, while nonimmunized controls showed\ntypical symptoms. In the subcutaneous-inoculation model, monkeys\nimmunized with LC16m8 showed no symptoms of monkeypox except for a mild\nulcer at the site of monkeypox virus inoculation, and those immunized\nwith Lister showed no symptoms of monkeypox, while nonimmunized\ncontrols showed lethal and typical symptoms. These results\nindicate that LC16m8 prevents lethal monkeypox in monkeys, and they\nsuggest that LC16m8 may induce protective immunity against\nsmallpox.<\/jats:p>","DOI":"10.1128\/jvi.02642-05","type":"journal-article","created":{"date-parts":[[2006,5,12]],"date-time":"2006-05-12T18:27:37Z","timestamp":1147458457000},"page":"5179-5188","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":125,"title":["LC16m8, a Highly Attenuated Vaccinia Virus Vaccine Lacking Expression of the Membrane Protein B5R, Protects Monkeys from Monkeypox"],"prefix":"10.1128","volume":"80","author":[{"given":"Masayuki","family":"Saijo","sequence":"first","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Ami","sequence":"additional","affiliation":[{"name":"Tokyo,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Suzaki","sequence":"additional","affiliation":[{"name":"Tokyo,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyo","family":"Nagata","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious\rDisease Pathology, Department of Pathology, National\rInstitute of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Iwata","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious\rDisease Pathology, Department of Pathology, National\rInstitute of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious\rDisease Pathology, Department of Pathology, National\rInstitute of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoko","family":"Ogata","sequence":"additional","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuetsu","family":"Fukushi","sequence":"additional","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsutaro","family":"Sata","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious\rDisease Pathology, Department of Pathology, National\rInstitute of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Kurata","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious\rDisease Pathology, Department of Pathology, National\rInstitute of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Kurane","sequence":"additional","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[{"name":"Special Pathogens Laboratory, Department of Virology\r1 Laboratory of Animal Experimentation"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2003.11.053"},{"key":"e_1_3_2_3_2","first-page":"106","volume":"53","year":"2004","unstructured":"CDC. 2004 . 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