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Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL\/6, BALB\/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.<\/jats:p>","DOI":"10.1128\/jvi.02667-05","type":"journal-article","created":{"date-parts":[[2006,5,12]],"date-time":"2006-05-12T18:27:37Z","timestamp":1147458457000},"page":"5523-5530","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Epitopes Expressed in Different Adenovirus Capsid Proteins Induce Different Levels of Epitope-Specific Immunity"],"prefix":"10.1128","volume":"80","author":[{"given":"Anja","family":"Krause","sequence":"first","affiliation":[{"name":"Department of Genetic Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju H.","family":"Joh","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil R.","family":"Hackett","sequence":"additional","affiliation":[{"name":"Belfer Gene Therapy Core Facility, Weill Medical College of Cornell University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Roelvink","sequence":"additional","affiliation":[{"name":"GenVec, Inc., Gaithersburg, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph T.","family":"Bruder","sequence":"additional","affiliation":[{"name":"GenVec, Inc., Gaithersburg, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Wickham","sequence":"additional","affiliation":[{"name":"GenVec, Inc., Gaithersburg, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imre","family":"Kovesdi","sequence":"additional","affiliation":[{"name":"GenVec, Inc., Gaithersburg, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald G.","family":"Crystal","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine"},{"name":"Belfer Gene Therapy Core Facility, Weill Medical College of Cornell University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Worgall","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine"},{"name":"Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.149"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.157"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-1-133"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.2.392"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.14.6893-6899.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9130-9136.1999"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.3.2388-2397.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2000.0203"},{"key":"e_1_3_2_10_2","first-page":"376","volume":"2","year":"2000","unstructured":"Hackett, N. 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