{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:03:30Z","timestamp":1791219810583,"version":"4.1.0"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,6,15]],"date-time":"2014-06-15T00:00:00Z","timestamp":1402790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2014,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Avian H7 influenza viruses are recognized as potential pandemic viruses, as personnel often become infected during poultry outbreaks. H7 infections in humans typically cause mild conjunctivitis; however, the H7N9 outbreak in the spring of 2013 has resulted in severe respiratory disease. To date, no H7 viruses have acquired the ability for sustained transmission among humans. Airborne transmission is considered a requirement for the emergence of pandemic influenza, and advanced knowledge of the molecular changes or signature required for transmission would allow early identification of pandemic vaccine seed stocks, screening and stockpiling of antiviral compounds, and eradication efforts focused on flocks harboring threatening viruses. Thus, we sought to determine if a highly pathogenic influenza A H7N1 (A\/H7N1) virus with no history of human infection could become capable of airborne transmission among ferrets. We show that after 10 serial passages, A\/H7N1 developed the ability to be transmitted to cohoused and airborne contact ferrets. Four amino acid mutations (PB2 T81I, NP V284M, and M1 R95K and Q211K) in the internal genes and a minimal amino acid mutation (K\/R313R) in the stalk region of the hemagglutinin protein were associated with airborne transmission. Furthermore, transmission was not associated with loss of virulence. These findings highlight the importance of the internal genes in host adaptation and suggest that natural isolates carrying these mutations be further evaluated. Our results demonstrate that a highly pathogenic avian H7 virus can become capable of airborne transmission in a mammalian host, and they support ongoing surveillance and pandemic H7 vaccine development.<\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The major findings of this report are that a highly pathogenic strain of H7N1 avian influenza virus can be adapted to become capable of airborne transmission in mammals without mutations altering receptor specificity. Changes in receptor specificity have been shown to play a role in the ability of avian influenza viruses to cross the species barrier, and these changes are assumed to be essential. The work reported here challenges this paradigm, at least for the influenza viruses of the H7 subtype, which have recently become the focus of major attention, as they have crossed to humans.\n          <\/jats:p>","DOI":"10.1128\/jvi.02765-13","type":"journal-article","created":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T05:13:22Z","timestamp":1396502002000},"page":"6623-6635","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Airborne Transmission of Highly Pathogenic H7N1 Influenza Virus in Ferrets"],"prefix":"10.1128","volume":"88","author":[{"given":"Troy C.","family":"Sutton","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Finch","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Angel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Capua","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Cattoli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Monne","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland College of Veterinary Medicine, Maryland Campus, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1648","volume-title":"Fields virology","author":"Palese P","year":"2007","unstructured":"PalesePShawML. 2007. 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Evaluating the safety and immune response of an H7N7 vaccine in healthy adults. http:\/\/www.clinicaltrials.gov\/ct2\/show\/NCT01534468."},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60938-1"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1371\/currents.RRN1011"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001081"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.2008\/004606-0"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02765-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02765-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:50:54Z","timestamp":1646430654000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02765-13"}},"subtitle":[],"editor":[{"given":"T. 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