{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:47:57Z","timestamp":1791128877191,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family\n            <jats:named-content content-type=\"genus-species\">Bunyaviridae<\/jats:named-content>\n            , but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus\n            <jats:named-content content-type=\"genus-species\">Phlebovirus<\/jats:named-content>\n            . Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus\n            <jats:named-content content-type=\"genus-species\">Phlebovirus<\/jats:named-content>\n            . The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.\n          <\/jats:p>","DOI":"10.1128\/jvi.02845-12","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T01:27:56Z","timestamp":1358386076000},"page":"3719-3728","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":108,"title":["Characterization of the Bhanja Serogroup Viruses (Bunyaviridae): a Novel Species of the Genus Phlebovirus and Its Relationship with Other Emerging Tick-Borne Phleboviruses"],"prefix":"10.1128","volume":"87","author":[{"given":"Keita","family":"Matsuno","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Weisend","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia P. A.","family":"Travassos da Rosa","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Anzick","sequence":"additional","affiliation":[{"name":"Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dahlstrom","sequence":"additional","affiliation":[{"name":"Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen F.","family":"Porcella","sequence":"additional","affiliation":[{"name":"Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Dorward","sequence":"additional","affiliation":[{"name":"Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Jie","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Tesh","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Ebihara","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"725","volume-title":"Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses","author":"Plyusnin A","year":"2011","unstructured":"PlyusninA BeatyBJ ElliottRM GoldbachR KormelinkR LundkvistA SchmaljohnCS TeshRB . 2011. Family Bunyaviridae, p 725\u2013741. In KingAMQ AdamsMJ CarstensEB LefkowitzEJ (ed), Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses, 1st ed. Elsevier, London, United Kingdom.","edition":"1"},{"key":"e_1_3_3_3_2","first-page":"1741","volume-title":"Fields virology","author":"Schmaljohn CS","year":"2007","unstructured":"SchmaljohnCS NicholST . 2007. Bunyaviridae, p 1741\u20131789. In KnipeDM HowleyPM GriffinDE LambRA MartinMA RoizmanB StrausSE (ed), Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"5"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002369"},{"key":"e_1_3_3_5_2","first-page":"864","article-title":"A novel bunyavirus in China","volume":"365","author":"Magiorkinis G","year":"2011","unstructured":"MagiorkinisG . 2011. A novel bunyavirus in China. N. Engl. J. Med. 365:864\u2013865.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1010095"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1803.111905"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203378"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1810.120835"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02448-05"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-007-1069-z"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02275-10"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2010.06.020"},{"key":"e_1_3_3_14_2","first-page":"67","article-title":"Isolation of Bhanja virus from ticks of the genus Haemaphysalis in southeast Bulgaria and presence of antibodies in pastured sheep","volume":"25","author":"Pavlov P","year":"1978","unstructured":"PavlovP Rosick\u00fdB Hub\u00e1lekZ DanielM B\u00e1rdosV Min\u00e1rJ Juricov\u00e1Z . 1978. Isolation of Bhanja virus from ticks of the genus Haemaphysalis in southeast Bulgaria and presence of antibodies in pastured sheep. Folia Parasitol. 25:67\u201373.","journal-title":"Folia Parasitol."},{"key":"e_1_3_3_15_2","first-page":"661","article-title":"Kismayo virus\u2014a representative of the Bhanja antigenic group","volume":"24","author":"Butenko AM","year":"1979","unstructured":"ButenkoAM GromashevskyVL LvovDK PopovVL . 1979. Kismayo virus\u2014a representative of the Bhanja antigenic group. Vop. Virus. 24:661\u2013665.","journal-title":"Vop. Virus."},{"key":"e_1_3_3_16_2","first-page":"183","article-title":"For\u00e9cariah virus, a new representative of the Bhanja antigenic group, isolated in the Republic of Guinea","volume":"79","author":"Boiro I","year":"1986","unstructured":"BoiroI LomonossovNN MalenkoGV BaldeC BahA . 1986. For\u00e9cariah virus, a new representative of the Bhanja antigenic group, isolated in the Republic of Guinea. Bull. Soc. Pathol. Exot. Filiales 79:183\u2013186. (In French.)","journal-title":"Bull. Soc. Pathol. Exot. Filiales"},{"key":"e_1_3_3_17_2","first-page":"139","article-title":"Isolation of Bhanja virus in Romania","volume":"49","author":"Ungureanu A","year":"1990","unstructured":"UngureanuA PopoviciV C\u0103t\u0103na\u015fF Ioni\u0163\u0103I TutoveanuA SaftaM . 1990. Isolation of Bhanja virus in Romania. Arch. Roum. Pathol. Exp. Microbiol. 49:139\u2013145.","journal-title":"Arch. Roum. Pathol. Exp. Microbiol."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01311000"},{"key":"e_1_3_3_19_2","first-page":"526","article-title":"Bhanja virus (Bunyaviridae) isolated from Dermacentor marginatus ticks in Czechoslovakia","volume":"32","author":"Hub\u00e1lek Z","year":"1987","unstructured":"Hub\u00e1lekZ MittermayerT HalouzkaJ . 1987. Bhanja virus (Bunyaviridae) isolated from Dermacentor marginatus ticks in Czechoslovakia. Acta Virol. 32:526.","journal-title":"Acta Virol."},{"key":"e_1_3_3_20_2","first-page":"77","article-title":"Geographic distribution of Bhanja virus","volume":"34","author":"Hub\u00e1lek Z","year":"1987","unstructured":"Hub\u00e1lekZ . 1987. Geographic distribution of Bhanja virus. Folia Parasitol. 34:77\u201386.","journal-title":"Folia Parasitol."},{"key":"e_1_3_3_21_2","first-page":"793","article-title":"Bhanja virus: a new arbovirus from ticks Haemaphysalis intermedia Warburton and Nuttall, 1909, in Orissa, India","volume":"57","author":"Shah KV","year":"1969","unstructured":"ShahKV WorkTH . 1969. Bhanja virus: a new arbovirus from ticks Haemaphysalis intermedia Warburton and Nuttall, 1909, in Orissa, India. Indian J. Med. Res. 57:793\u2013798.","journal-title":"Indian J. Med. Res."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1970.19.103"},{"key":"e_1_3_3_23_2","first-page":"82","article-title":"Isolation of Bhanja virus from Dermacentor marginatus Suiz (1776) ticks in Armenian SSR","volume":"2","author":"Zakaryan VA","year":"1974","unstructured":"ZakaryanVA GromashevskyVL ChubkovaAI AkopyanGS SkvortsevaTM . 1974. Isolation of Bhanja virus from Dermacentor marginatus Suiz (1776) ticks in Armenian SSR. Sborn. Trud. Ekol. Virus. 2:82\u201384.","journal-title":"Sborn. Trud. Ekol. Virus."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1977.26.1003"},{"key":"e_1_3_3_25_2","first-page":"273","article-title":"Laboratory infections with Bhanja virus","author":"Punda V","year":"1980","unstructured":"PundaV BeusI CalisherCH Vesenjak-HirjanJ . 1980. Laboratory infections with Bhanja virus. Zentralbl. Bakteriol. Suppl 9:273\u2013275.","journal-title":"Zentralbl. Bakteriol."},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1975.24.1040"},{"key":"e_1_3_3_27_2","first-page":"297","volume-title":"Arboviruses in the Mediterranean countries: 6th FEMS symposium","author":"Vesenjak-Hirjan J","year":"1980","unstructured":"Vesenjak-HirjanJ CalisherCH BeusI MartonE . 1980. First natural clinical human Bhanja virus infection, p 297\u2013301. In Vesenjak-HirjanJ PorterfieldJS Arslanagi\u0107E (ed), Arboviruses in the Mediterranean countries: 6th FEMS symposium. Fischer, Stuttgart, Germany."},{"key":"e_1_3_3_28_2","first-page":"227","article-title":"Incidence of hemagglutination-inhibiting antibodies for Bhanja virus in humans along the north-west border of Yugoslavia","volume":"265","author":"Punda V","year":"1987","unstructured":"PundaV RopacD Vesenjak-HirjanJ . 1987. Incidence of hemagglutination-inhibiting antibodies for Bhanja virus in humans along the north-west border of Yugoslavia. Zentralbl. Bakteriol. Mikrobiol. Hyg. A 265:227\u2013234.","journal-title":"Zentralbl. Bakteriol. Mikrobiol. Hyg. A"},{"key":"e_1_3_3_29_2","first-page":"355","article-title":"Antibodies against the Bhanja virus in agricultural workers in Eastern Slovakia","volume":"35","author":"Mittermayer T","year":"1986","unstructured":"MittermayerT Hub\u00e1lekZ . 1986. Antibodies against the Bhanja virus in agricultural workers in Eastern Slovakia. Cesk. Epidemiol. Mikrobiol. Imunol. 35:355\u2013359. (In Slovak.)","journal-title":"Cesk. Epidemiol. Mikrobiol. Imunol."},{"key":"e_1_3_3_30_2","first-page":"316","volume-title":"The arthropod-borne viruses of vertebrates","author":"Theiler M","year":"1973","unstructured":"TheilerM DownsWG . 1973. The arthropod-borne viruses of vertebrates, 1st ed, p 316\u2013338. Yale University Press, New Haven, CT.","edition":"1"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000150399"},{"key":"e_1_3_3_32_2","first-page":"797","volume-title":"Diagnostic procedures for viral, rickettsial and chlamydial infections","author":"Beaty BJ","year":"1989","unstructured":"BeatyBJ CalisherCH ShopeRE . 1989. Arboviruses, p 797\u2013855. In SchmidtNJ EmmonsRW (ed), Diagnostic procedures for viral, rickettsial and chlamydial infections, 6th ed. American Public Health Association, Washington, DC.","edition":"6"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1983.32.1164"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-10"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.8.2019"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1301.060837"},{"key":"e_1_3_3_37_2","unstructured":"454 Life Sciences. October 2009. Rapid library preparation method manual: GS FLX titanium series. Roche Diagnostics Mannheim Germany."},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq427"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl677"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02719-12"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80407-0"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90430-2"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90087-R"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.1.218-225.1997"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.23.12200-12210.2002"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00767-07"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001760107"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002030"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00612-12"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9798-9806.2004"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-012-0785-y"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-6-1345"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2012.04.003"},{"key":"e_1_3_3_56_2","first-page":"9227","article-title":"Gouleako virus isolated from West African mosquitoes constitutes a proposed novel genus in the family","volume":"85","author":"Marklewitz M","year":"2012","unstructured":"MarklewitzM HandrickS GrasseW KurthA LukashevA DrostenC EllerbrokH LeendertzFH PauliG JunglenS . 2012. Gouleako virus isolated from West African mosquitoes constitutes a proposed novel genus in the family Bunyaviridae. 85:9227\u20139234.","journal-title":"Bunyaviridae"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02845-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02845-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:35:10Z","timestamp":1646494510000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02845-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1128\/JVI.02845-12"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02845-12","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4]]},"assertion":[{"value":"2012-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}