{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:52:53Z","timestamp":1791251573374,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T00:00:00Z","timestamp":1458000000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UC7 5UAI094660"],"award-info":[{"award-number":["UC7 5UAI094660"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["UC7 5UAI094660"],"award-info":[{"award-number":["UC7 5UAI094660"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["K08DC011540"],"award-info":[{"award-number":["K08DC011540"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2016,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Approximately one-third of Lassa virus (LASV)-infected patients develop sensorineural hearing loss (SNHL) in the late stages of acute disease or in early convalescence. With 500,000 annual cases of Lassa fever (LF), LASV is a major cause of hearing loss in regions of West Africa where LF is endemic. To date, no animal models exist that depict the human pathology of LF with associated hearing loss. Here, we aimed to develop an animal model to study LASV-induced hearing loss using human isolates from a 2012 Sierra Leone outbreak. We have recently established a murine model for LF that closely mimics many features of human disease. In this model, LASV isolated from a lethal human case was highly virulent, while the virus isolated from a nonlethal case elicited mostly mild disease with moderate mortality. More importantly, both viruses were able to induce SNHL in surviving animals. However, utilization of the nonlethal, human LASV isolate allowed us to consistently produce large numbers of survivors with hearing loss. Surviving mice developed permanent hearing loss associated with mild damage to the cochlear hair cells and, strikingly, significant degeneration of the spiral ganglion cells of the auditory nerve. Therefore, the pathological changes in the inner ear of the mice with SNHL supported the phenotypic loss of hearing and provided further insights into the mechanistic cause of LF-associated hearing loss.<\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Sensorineural hearing loss is a major complication for LF survivors. The development of a small-animal model of LASV infection that replicates hearing loss and the clinical and pathological features of LF will significantly increase knowledge of pathogenesis and vaccine studies. In addition, such a model will permit detailed characterization of the hearing loss mechanism and allow for the development of appropriate diagnostic approaches and medical care for LF patients with hearing impairment.\n          <\/jats:p>","DOI":"10.1128\/jvi.02948-15","type":"journal-article","created":{"date-parts":[[2015,12,31]],"date-time":"2015-12-31T02:54:03Z","timestamp":1451530443000},"page":"2920-2927","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Animal Model of Sensorineural Hearing Loss Associated with Lassa Virus Infection"],"prefix":"10.1128","volume":"90","author":[{"given":"Nadezhda E.","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Galveston National Laboratory, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Ronca","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Koma","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey V.","family":"Seregin","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly T.","family":"Dineley","sequence":"additional","affiliation":[{"name":"Metabiota, Washington, DC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milagros","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida G.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanon N.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph N.","family":"Fair","sequence":"additional","affiliation":[{"name":"Metabiota, Washington, DC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Wauquier","sequence":"additional","affiliation":[{"name":"Metabiota, Freetown, Sierra Leone"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bayon","family":"Bockarie","sequence":"additional","affiliation":[{"name":"Metabiota, Freetown, Sierra Leone"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheik Humarr","family":"Khan","sequence":"additional","affiliation":[{"name":"Ministry of Health and Sanitation, Freetown, Sierra Leone"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Makishima","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slobodan","family":"Paessler","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA"},{"name":"Galveston National Laboratory, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"52","article-title":"High Lassa Fever activity in northern part of Edo State, Nigeria: reanalysis of confirmatory test results","volume":"17","author":"Eze KC","year":"2010","unstructured":"Eze KC, Salami TAT, Eze IC, Pogoson AE, Omordia N, Ugochukwu MO. 2010. High Lassa Fever activity in northern part of Edo State, Nigeria: reanalysis of confirmatory test results. Afr J Health Sci 17:52\u201356.","journal-title":"Afr J Health Sci"},{"key":"e_1_3_2_3_2","first-page":"1283","volume-title":"Fields virology","author":"Buchmeier MJ","year":"2013","unstructured":"Buchmeier MJ, de la Torre JC, Peters CJ. 2013. Arenaviridae, p 1283\u20131303. In Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR, Roizman B (ed), Fields virology, 6th ed, vol 2. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.445"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1990.03450160063030"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1990.03450160089037"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/2331216514541361"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/0883073813486295"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.126297"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(97)70248-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e318067bd42"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/14992027.2012.721935"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3109\/00207458509149137"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-011-6011-3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.01.017"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.spen.2012.02.002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01433-13"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2015.02.012"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06284-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003359900453"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-0709(88)80041-3"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20260"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/019459989210600303"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-010-1370-4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198601023140104"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.11.023"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2232366100"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4102031"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7673"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.2.609"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2834"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M910149199"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200007)30:7<1851::AID-IMMU1851>3.0.CO;2-C"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802130105"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.4.2158"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02948-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02948-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:17:48Z","timestamp":1646489868000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02948-15"}},"subtitle":[],"editor":[{"given":"S. R.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,3,15]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,3,15]]}},"alternative-id":["10.1128\/JVI.02948-15"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02948-15","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,15]]},"assertion":[{"value":"2015-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}