{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:56:53Z","timestamp":1785549413824,"version":"3.56.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T00:00:00Z","timestamp":1460592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Key Basic Research Plan","award":["2014CB542700"],"award-info":[{"award-number":["2014CB542700"]}]},{"name":"Huazhong Agricultural University Scientific and Technological Self-innovation Foundation","award":["2012RC008"],"award-info":[{"award-number":["2012RC008"]}]},{"name":"Huazhong Agricultural University Scientific and Technological Self-innovation Foundation","award":["2013PY031"],"award-info":[{"award-number":["2013PY031"]}]},{"name":"Huazhong Agricultural University Scientific and Technological Self-innovation Foundation","award":["2662015JQ003"],"award-info":[{"award-number":["2662015JQ003"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31225027"],"award-info":[{"award-number":["31225027"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31372440"],"award-info":[{"award-number":["31372440"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Porcine reproductive and respiratory syndrome virus (PRRSV) RNA endoribonuclease nsp11 belongs to the XendoU superfamily and plays a crucial role in arterivirus replication. Here, we report the first crystal structure of the arterivirus nsp11 protein from PRRSV, which exhibits a unique structure and assembles into an asymmetric dimer whose structure is completely different from the hexameric structure of coronavirus nsp15. However, the structures of the PRRSV nsp11 and coronavirus nsp15 catalytic domains were perfectly superimposed, especially in the \u201cactive site loop\u201d (His129 to His144) and \u201csupporting loop\u201d (Val162 to Thr179) regions. Importantly, our biochemical data demonstrated that PRRSV nsp11 exists mainly as a dimer in solution. Mutations of the major dimerization site determinants (Ser74 and Phe76) in the dimerization interface destabilized the dimer in solution and severely diminished endoribonuclease activity, indicating that the dimer is the biologically functional unit. In the dimeric structure, the active site loop and supporting loop are packed against one another and stabilized by monomer-monomer interactions. These findings may help elucidate the mechanism underlying arterivirus replication and may represent great potential for the development of antiviral drugs.<\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family\n            <jats:named-content content-type=\"genus-species\">Arteriviridae<\/jats:named-content>\n            , order\n            <jats:named-content content-type=\"genus-species\">Nidovirales<\/jats:named-content>\n            . PRRSV is a major agent of respiratory diseases in pigs, causing tremendous economic losses to the swine industry worldwide. The PRRSV nsp11 endoribonuclease plays a vital role in arterivirus replication, but its precise roles and mechanisms of action are poorly understood. Here, we report the first dimeric structure of the arterivirus nsp11 from PRRSV at 2.75-\u212b resolution. Structural and biochemical experiments demonstrated that nsp11 exists mainly as a dimer in solution and that nsp11 may be fully active as a dimer. Mutagenesis and structural analysis revealed NendoU active site residues, which are conserved throughout the order\n            <jats:named-content content-type=\"genus-species\">Nidovirales<\/jats:named-content>\n            (families\n            <jats:named-content content-type=\"genus-species\">Arteriviridae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Coronaviridae<\/jats:named-content>\n            ) and the major determinants of dimerization (Ser74 and Phe76) in\n            <jats:named-content content-type=\"genus-species\">Arteriviridae<\/jats:named-content>\n            . Importantly, these findings may provide a new structural basis for antiviral drug development.\n          <\/jats:p>","DOI":"10.1128\/jvi.03065-15","type":"journal-article","created":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T04:14:07Z","timestamp":1456373647000},"page":"4579-4592","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of Porcine Reproductive and Respiratory Syndrome Virus nsp11"],"prefix":"10.1128","volume":"90","author":[{"given":"Yuejun","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 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