{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:56:18Z","timestamp":1791255378813,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,6,15]],"date-time":"2013-06-15T00:00:00Z","timestamp":1371254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toscana virus (TOSV) is a phlebovirus, of the\n            <jats:named-content content-type=\"genus-species\">Bunyaviridae<\/jats:named-content>\n            family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-\u03b2) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol.\n            <jats:bold>92:<\/jats:bold>\n            71\u201379, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-\u03b2 promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (\u0394NSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-\u03b2 promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response.\n          <\/jats:p>","DOI":"10.1128\/jvi.03129-12","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T06:59:59Z","timestamp":1365058799000},"page":"6660-6667","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I"],"prefix":"10.1128","volume":"87","author":[{"given":"Gianni","family":"Gori-Savellini","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnologies, University of Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Valentini","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies, University of Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Cusi","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies, University of Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125676"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.778-809.2001"},{"key":"e_1_3_1_4_2","first-page":"54","article-title":"RNA recognition and signal transduction by RIG-like receptors","volume":"227","author":"Yoneyama M","year":"2008","unstructured":"YoneyamaM FujitaT. 2008. 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