{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T20:26:37Z","timestamp":1789849597765,"version":"4.0.1"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Rabies, as the oldest known infectious disease, remains a serious threat to public health worldwide. The eukaryotic cytosolic chaperonin TRiC\/CCT complex facilitates the folding of proteins through ATP hydrolysis. Here, we investigated the expression, cellular localization, and function of neuronal CCT\u03b3 during neurotropic rabies virus (RABV) infection using mouse N2a cells as a model. Following RABV infection, 24 altered proteins were identified by using two-dimensional electrophoresis and mass spectrometry, including 20 upregulated proteins and 4 downregulated proteins. In mouse N2a cells infected with RABV or cotransfected with RABV genes encoding nucleoprotein (N) and phosphoprotein (P), confocal microscopy demonstrated that upregulated cellular CCT\u03b3 was colocalized with viral proteins N and P, which formed a hollow cricoid inclusion within the region around the nucleus. These inclusions, which correspond to Negri bodies (NBs), did not form in mouse N2a cells only expressing the viral protein N or P. Knockdown of CCT\u03b3 by lentivirus-mediated RNA interference led to significant inhibition of RABV replication. These results demonstrate that the complex consisting of viral proteins N and P recruits CCT\u03b3 to NBs and identify the chaperonin CCT\u03b3 as a host factor that facilitates intracellular RABV replication. This work illustrates how viruses can utilize cellular chaperonins and compartmentalization for their own benefit.<\/jats:p>","DOI":"10.1128\/jvi.03186-12","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T08:36:26Z","timestamp":1367483786000},"page":"7608-7621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Cellular Chaperonin CCT\u03b3 Contributes to Rabies Virus Replication during Infection"],"prefix":"10.1128","volume":"87","author":[{"given":"Jinyang","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjin","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhen","family":"Ruan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-387040-7.00003-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.11.10"},{"key":"e_1_3_3_4_2","unstructured":"WHO. September 2012. Rabies: fact sheet no. 99. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/mediacentre\/factsheets\/fs099\/en\/. Accessed 26 December 2012."},{"key":"e_1_3_3_5_2","first-page":"88","article-title":"Contributo allo studio dell'eziologia della rabbia","volume":"2","author":"Negri A","year":"1903","unstructured":"NegriA. 1903. Contributo allo studio dell'eziologia della rabbia. Boll. Soc. Med. Chir. Pavia 2:88\u2013115.","journal-title":"Boll. Soc. Med. Chir. Pavia"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200220"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-97-23641"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-9-2137"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1996.tb00893.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2080"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.66.539"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06501-11"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00554-09"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00213-10"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700544-MCP200"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.001651"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700520-MCP200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800541-MCP200"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051630298"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.19.12554-12565.2005"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2011.09.003"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280601178226"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1010.10003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701007"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr200276u"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90923-D"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000315"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.03.015"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.000570"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01085"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.1.99"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.10.026"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00813-10"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.6.2526-2534.2001"},{"key":"e_1_3_3_35_2","first-page":"33","article-title":"Epstein-Barr virus-encoded nuclear protein EBNA-3 interacts with the epsilon-subunit of the T-complex protein 1 chaperonin complex","volume":"2","author":"Kashuba E","year":"1999","unstructured":"KashubaE PokrovskajaK KleinG SzekelyL. 1999. Epstein-Barr virus-encoded nuclear protein EBNA-3 interacts with the epsilon-subunit of the T-complex protein 1 chaperonin complex. J. Hum. Virol. 2:33\u201337.","journal-title":"J. Hum. Virol"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700396-MCP200"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr900488q"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100479"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200011)21:17<3666::AID-ELPS3666>3.0.CO;2-6"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012465330-6\/50003-8"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19074-0"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-11-2889"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/843206"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.9.3141-3151.2003"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1464"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/358249a0"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5267.1497"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)00190-0"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00278.2004"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.3.435"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205931"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.20836"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500090-MCP200"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.03186-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.03186-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:28:36Z","timestamp":1646494116000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.03186-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":53,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1128\/JVI.03186-12"],"URL":"https:\/\/doi.org\/10.1128\/jvi.03186-12","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]},"assertion":[{"value":"2012-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}