{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:06:21Z","timestamp":1792735581417,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Many mucosal factors in the female genital tract (FGT) have been associated with HIV susceptibility, but little is known about their anatomical distribution in the FGT compartments. This study comprehensively characterized global immune factor expression in different tissue sites of the lower and upper FGT by using a systems biology approach. Tissue sections from the ectocervix, endocervix, and endometrium from seven women who underwent hysterectomy were analyzed by a combination of quantitative mass spectrometry and immunohistochemical staining. Of the &gt;1,000 proteins identified, 281 were found to be differentially abundant in different tissue sites. Hierarchical clustering identified four major functional pathways distinguishing compartments, including innate immune pathways (acute-phase response, LXR\/RXR) and development (RhoA signaling, gluconeogenesis), which were enriched in the ectocervix\/endocervix and endometrium, respectively. Immune factors important for HIV susceptibility, including antiproteases, immunoglobulins, complement components, and antimicrobial factors, were most abundant in the ectocervix\/endocervix, while the endometrium had a greater abundance of certain factors that promote HIV replication. Immune factor abundance is heterogeneous throughout the FGT and shows unique immune microenvironments for HIV based on the exposure site. This may have important implications for early events in HIV transmission and site-specific susceptibility to HIV in the FGT.<\/jats:p>","DOI":"10.1128\/jvi.03347-12","type":"journal-article","created":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T16:49:11Z","timestamp":1362156551000},"page":"5141-5150","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["A Systems Biology Examination of the Human Female Genital Tract Shows Compartmentalization of Immune Factor Expression"],"prefix":"10.1128","volume":"87","author":[{"given":"Adam","family":"Burgener","sequence":"first","affiliation":[{"name":"University of Manitoba, Department of Medical Microbiology, Winnipeg, Manitoba, Canada"},{"name":"Proteomics Group, National Laboratory for HIV Immunology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelie","family":"Tjernlund","sequence":"additional","affiliation":[{"name":"Karolinska Institutet, Department of Medicine Solna, Center for Molecular Medicine, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tove","family":"Kaldensjo","sequence":"additional","affiliation":[{"name":"Karolinska Institutet, Department of Medicine Solna, Center for Molecular Medicine, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Abou","sequence":"additional","affiliation":[{"name":"National Laboratory for HIV Immunology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart","family":"McCorrister","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Core Facility, National Microbiology Lab, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garrett R.","family":"Westmacott","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Core Facility, National Microbiology Lab, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzie","family":"Mogk","sequence":"additional","affiliation":[{"name":"University of Manitoba, Department of Medical Microbiology, Winnipeg, Manitoba, Canada"},{"name":"Proteomics Group, National Laboratory for HIV Immunology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Ambrose","sequence":"additional","affiliation":[{"name":"University of Manitoba, Department of Medical Microbiology, Winnipeg, Manitoba, Canada"},{"name":"Proteomics Group, National Laboratory for HIV Immunology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Broliden","sequence":"additional","affiliation":[{"name":"Karolinska Institutet, Department of Medicine Solna, Center for Molecular Medicine, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blake","family":"Ball","sequence":"additional","affiliation":[{"name":"University of Manitoba, Department of Medical Microbiology, Winnipeg, Manitoba, Canada"},{"name":"Proteomics Group, National Laboratory for HIV Immunology, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"},{"name":"Mass Spectrometry Core Facility, National Microbiology Lab, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"},{"name":"University of Nairobi, Department of Medical Microbiology, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"UNAIDS. 2011. 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