{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:58:09Z","timestamp":1790517489889,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Enterovirus 71 (EV71) is an important emerging human pathogen with a global distribution and presents a disease pattern resembling poliomyelitis with seasonal epidemics that include cases of severe neurological complications, such as acute flaccid paralysis. EV71 is a member of the<jats:named-content content-type=\"genus-species\">Picornaviridae<\/jats:named-content>family, which consists of icosahedral, nonenveloped, single-stranded RNA viruses. Here we report structures derived from X-ray crystallography and cryoelectron microscopy (cryo-EM) for the 1095 strain of EV71, including a putative precursor in virus assembly, the procapsid, and the mature virus capsid. The cryo-EM map of the procapsid provides new structural information on portions of the capsid proteins VP0 and VP1 that are disordered in the higher-resolution crystal structures. Our structures solved from virus particles in solution are largely in agreement with those from prior X-ray crystallographic studies; however, we observe small but significant structural differences for the 1095 procapsid compared to a structure solved in a previous study (X. Wang, W. Peng, J. Ren, Z. Hu, J. Xu, Z. Lou, X. Li, W. Yin, X. Shen, C. Porta, T. S. Walter, G. Evans, D. Axford, R. Owen, D. J. Rowlands, J. Wang, D. I. Stuart, E. E. Fry, and Z. Rao, Nat. Struct. Mol. Biol. 19:424\u2013429, 2012) for a different strain of EV71. For both EV71 strains, the procapsid is significantly larger in diameter than the mature capsid, unlike in any other picornavirus. Nonetheless, our results demonstrate that picornavirus capsid expansion is possible without RNA encapsidation and that picornavirus assembly may involve an inward radial collapse of the procapsid to yield the native virion.<\/jats:p>","DOI":"10.1128\/jvi.03519-12","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T08:36:26Z","timestamp":1367483786000},"page":"7637-7645","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Structures of the Procapsid and Mature Virion of Enterovirus 71 Strain 1095"],"prefix":"10.1128","volume":"87","author":[{"given":"Javier O.","family":"Cifuente","sequence":"first","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua D.","family":"Yoder","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin L.","family":"Shingler","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Carnegie","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Yoder","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Ashley","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander M.","family":"Makhov","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James F.","family":"Conway","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Hafenstein","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/129.3.304"},{"key":"e_1_3_2_3_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70194-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00630-09"},{"key":"e_1_3_2_6_2","first-page":"939","article-title":"Emerging viral infections of the central nervous system: part 1","volume":"66","author":"Tyler KL","year":"2009","unstructured":"TylerKL. 2009. Emerging viral infections of the central nervous system: part 1. Arch. Neurol. 66:939\u2013948.","journal-title":"Arch. Neurol"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.029371-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"254","DOI":"10.7883\/yoken.JJID.2009.254","article-title":"Genetic changes of coxsackievirus A16 and enterovirus 71 isolated from hand, foot, and mouth disease patients in Toyama, Japan between 1981 and 2007","volume":"62","author":"Iwai M","year":"2009","unstructured":"IwaiM MasakiA HasegawaS ObaraM HorimotoE NakamuraK TanakaY EndoK TanakaK UedaJ ShirakiK KurataT TakizawaT. 2009. Genetic changes of coxsackievirus A16 and enterovirus 71 isolated from hand, foot, and mouth disease patients in Toyama, Japan between 1981 and 2007. Jpn. J. Infect. Dis. 62:254\u2013259.","journal-title":"Jpn. J. Infect. Dis"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218713"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2255"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23287"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026237"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01019-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1961"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1992"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-162"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-6-141"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00297-11"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02226-12"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.301622"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01033-12"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.12.5.1181-1183.1973"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(77)90005-8"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.1.21"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90289-5"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1636"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.9.4067-4075.1990"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.022418-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2276"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767393007597"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2011.09.006"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1626"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0897-666"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0395-224"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(01)00201-5"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)88343-4"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444998002790"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.18.9780-9789.2003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2005.04.011"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00837-10"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767387011875"},{"key":"e_1_3_2_46_2","first-page":"353","volume-title":"International tables for crystallography","author":"Kleywegt GJ","year":"2001","unstructured":"KleywegtGJ ZouJY KjeldgaardM JonesTA. 2001. Around O, p 353\u2013356. In RossmannMG ArnoldE (ed), International tables for crystallography, vol F. Kluwer Academic Publishers, Dordrecht, The Netherlands."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444998003254"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927608080781"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2006.08.007"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2006.05.009"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2006.07.013"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2006.06.010"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-009-0026-3"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2002.5438"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020005"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02070-12"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.12.9743-9752.1997"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-9-26"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.010"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00640-12"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(68)90195-2"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.10.2.261-266.1972"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.39.2.341-347.1981"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.11.5350-5356.2002"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.03519-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.03519-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T12:02:55Z","timestamp":1688212975000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.03519-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":64,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1128\/JVI.03519-12"],"URL":"https:\/\/doi.org\/10.1128\/jvi.03519-12","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]},"assertion":[{"value":"2012-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}