{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T04:37:25Z","timestamp":1793162245307,"version":"4.5.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel H1N1 influenza virus emerged in 2009 (pH1N1) to become the first influenza pandemic of the 21st century. This virus is now cocirculating with highly pathogenic H5N1 avian influenza viruses in many parts of the world, raising concerns that a reassortment event may lead to highly pathogenic influenza strains with the capacity to infect humans more readily and cause severe disease. To investigate the virulence of pH1N1-H5N1 reassortant viruses, we created pH1N1 (A\/California\/04\/2009) viruses expressing individual genes from an avian H5N1 influenza strain (A\/Hong Kong\/483\/1997). Using several\n            <jats:italic>in vitro<\/jats:italic>\n            models of virus replication, we observed increased replication for a reassortant CA\/09 virus expressing the hemagglutinin (HA) gene of HK\/483 (CA\/09-483HA) relative to that of either parental CA\/09 virus or reassortant CA\/09 expressing other HK\/483 genes. This increased replication correlated with enhanced pathogenicity in infected mice similar to that of the parental HK\/483 strain. The serial passage of the CA\/09 parental virus and the CA\/09-483HA virus through primary human lung epithelial cells resulted in increased pathogenicity, suggesting that these viruses easily adapt to humans and become more virulent. In contrast, serial passage attenuated the parental HK\/483 virus\n            <jats:italic>in vitro<\/jats:italic>\n            and resulted in slightly reduced morbidity\n            <jats:italic>in vivo<\/jats:italic>\n            , suggesting that sustained replication in humans attenuates H5N1 avian influenza viruses. Taken together, these data suggest that reassortment between cocirculating human pH1N1 and avian H5N1 influenza strains will result in a virus with the potential for increased pathogenicity in mammals.\n          <\/jats:p>","DOI":"10.1128\/jvi.05582-11","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T10:13:06Z","timestamp":1316081586000},"page":"12262-12270","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Increased Pathogenicity of a Reassortant 2009 Pandemic H1N1 Influenza Virus Containing an H5N1 Hemagglutinin"],"prefix":"10.1128","volume":"85","author":[{"given":"Troy D.","family":"Cline","sequence":"first","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik A.","family":"Karlsson","sequence":"additional","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Freiden","sequence":"additional","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley J.","family":"Seufzer","sequence":"additional","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerold E.","family":"Rehg","sequence":"additional","affiliation":[{"name":"Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Webby","sequence":"additional","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey","family":"Schultz-Cherry","sequence":"additional","affiliation":[{"name":"Departments of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2010.06.008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813234106"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1637\/9422-061410-Reg.1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001136"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511120103"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000072"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1201-1306"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11772-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)11212-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.09.010"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0903810"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1477"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02717-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00268-2"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.5.3120-3128.1994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01678-06"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.06.003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605134103"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1177238"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01785-09"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.41.2.80"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08157"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01140-10"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000115"},{"key":"e_1_3_2_26_2","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed L. 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