{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:07:42Z","timestamp":1789164462756,"version":"build-2803163510"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T00:00:00Z","timestamp":1323907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Epstein-Barr Virus (EBV) is an ubiquitous human herpesvirus which can lead to infectious mononucleosis and different cancers. In immunocompromised individuals, this virus is a major cause for morbidity and mortality. Transplant patients who did not encounter EBV prior to immunosuppression frequently develop EBV-associated malignancies, but a prophylactic EBV vaccination might reduce this risk considerably. Virus-like particles (VLPs) mimic the structure of the parental virus but lack the viral genome. Therefore, VLPs are considered safe and efficient vaccine candidates. We engineered a dedicated producer cell line for EBV-derived VLPs. This cell line contains a genetically modified EBV genome which is devoid of all potential viral oncogenes but provides viral proteins essential for the assembly and release of VLPs via the endosomal sorting complex required for transport (ESCRT). Human B cells readily take up EBV-based VLPs and present viral epitopes in association with HLA molecules to T cells. Consequently, EBV-based VLPs are highly immunogenic and elicit humoral and strong CD8\n                    <jats:sup>+<\/jats:sup>\n                    and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell responses\n                    <jats:named-content content-type=\"genus-species\">in vitro<\/jats:named-content>\n                    and in a preclinical murine model\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Our findings suggest that VLP formulations might be attractive candidates to develop a safe and effective polyvalent vaccine against EBV.\n                  <\/jats:p>","DOI":"10.1128\/jvi.05598-11","type":"journal-article","created":{"date-parts":[[2011,10,12]],"date-time":"2011-10-12T22:31:46Z","timestamp":1318458706000},"page":"13105-13113","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["A Virus-Like Particle-Based Epstein-Barr Virus Vaccine"],"prefix":"10.1128","volume":"85","author":[{"given":"Romana","family":"Ruiss","sequence":"first","affiliation":[{"name":"Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, Marchioninistr. 25, D-81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Jochum","sequence":"additional","affiliation":[{"name":"Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, Marchioninistr. 25, D-81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Wanner","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universit\u00e4t, Department Biology I, Gro\u00dfhadernerstr. 2, D-82152 Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Reisbach","sequence":"additional","affiliation":[{"name":"Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, Marchioninistr. 25, D-81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Hammerschmidt","sequence":"additional","affiliation":[{"name":"Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, Marchioninistr. 25, D-81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Zeidler","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-Universit\u00e4t, ENT-Department, Marchioninistr. 15, D-81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"IARC monograph on the evaluation of carcinogenic risks to humans.","author":"Ablashi D. 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