{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T07:00:52Z","timestamp":1793170852101,"version":"4.5.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T00:00:00Z","timestamp":1323907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Candid#1 (Cd1) is an attenuated vaccine strain of Junin virus, the causative agent of Argentine hemorrhagic fever. Although several substitutions are present in Cd1, their importance for attenuation has not been established. We functionally characterized the substitutions present in the Cd1 glycoprotein (GP) and identified F427I in the transmembrane domain of the GP2 subunit as reducing infectivity in a reconstituted viral system. We further showed that this phenotype derives from the destabilization of the GP metastable conformation. Lastly, we identified an increased dependence of Cd1 GP on human transferrin receptor type 1 (hTfR-1) for entry, which may affect the tropism of the attenuated strain\n            <jats:italic>in vivo<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jvi.05616-11","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T05:58:09Z","timestamp":1317880689000},"page":"13457-13462","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Substitutions in the Glycoprotein (GP) of the Candid#1 Vaccine Strain of Junin Virus Increase Dependence on Human Transferrin Receptor 1 for Entry and Destabilize the Metastable Conformation of GP"],"prefix":"10.1128","volume":"85","author":[{"given":"Magali E.","family":"Droniou-Bonzom","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therese","family":"Reignier","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill E.","family":"Oldenburg","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex U.","family":"Cox","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin M.","family":"Exline","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Y.","family":"Rathbun","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M.","family":"Cannon","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1772"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00208-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00856-11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00276-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-78-7-1605"},{"issue":"2","key":"e_1_3_2_7_2","first-page":"118","article-title":"Towards a vaccine against Argentine hemorrhagic fever","volume":"25","author":"Barrera Oro J. G.","year":"1991","unstructured":"Barrera OroJ. G. McKeeK. T.Jr. 1991. Towards a vaccine against Argentine hemorrhagic fever. Bull. Pan Am. Health Organ. 25(2):118\u2013126.","journal-title":"Bull. Pan Am. Health Organ."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00761-09"},{"key":"e_1_3_2_10_2","first-page":"159","article-title":"Arenaviruses: protein structure and function","volume":"262","author":"Buchmeier M. J.","year":"2002","unstructured":"BuchmeierM. J. 2002. Arenaviruses: protein structure and function. Curr. Top. Microbiol. Immunol. 262:159\u2013173.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2079"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01321064"},{"key":"e_1_3_2_13_2","first-page":"86","article-title":"Lysosomotropic compounds inhibiting the multiplication of Junin virus","volume":"23","author":"Castilla V.","year":"1991","unstructured":"CastillaV. MersichS. E. DamonteE. B.. 1991. Lysosomotropic compounds inhibiting the multiplication of Junin virus. Rev. Argent. Microbiol. 23:86\u201389.","journal-title":"Rev. Argent. Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1076"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608678103"},{"key":"e_1_3_2_16_2","first-page":"1","article-title":"Reverse genetics of mononegavirales","volume":"283","author":"Conzelmann K. K.","year":"2004","unstructured":"ConzelmannK. K. 2004. Reverse genetics of mononegavirales. Curr. Top. Microbiol. Immunol. 283:1\u201341.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1057"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.777"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02102-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2007.10.010"},{"key":"e_1_3_2_22_2","first-page":"239","article-title":"Junin virus vaccines","volume":"263","author":"Enria D. A.","year":"2002","unstructured":"EnriaD. A. Barrero OroJ. G.. 2002. Junin virus vaccines. Curr. Top. Microbiol. Immunol. 263:239\u2013261.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00008-06"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01397-07"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)81140-J"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01313817"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-010-0450-2"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02308-10"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.4.2232-2239.1992"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.10.5979-5987.2005"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1086\/514211"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1996.12.811"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.03.003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90020-6"},{"issue":"62","key":"e_1_3_2_36_2","first-page":"2300","article-title":"Concerning the epidemic outbreak in Junin","volume":"30","author":"Parodi A. S.","year":"1958","unstructured":"ParodiA. S.. 1958. Concerning the epidemic outbreak in Junin. Dia Med. 30(62):2300\u20132301.","journal-title":"Dia Med."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05539"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.05.018"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.1.448-457.2001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.10.5140-5146.2002"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1998.14.1553"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02410-08"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01140-08"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00642-06"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.08.030"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.19.10783-10792.2004"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.05616-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.05616-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T16:22:52Z","timestamp":1646497372000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.05616-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,15]]},"references-count":45,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2011,12,15]]}},"alternative-id":["10.1128\/JVI.05616-11"],"URL":"https:\/\/doi.org\/10.1128\/jvi.05616-11","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,15]]},"assertion":[{"value":"2011-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}