{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T19:31:07Z","timestamp":1793734267833,"version":"4.6.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The arenavirus Z is a zinc-binding RING protein that has been implicated in multiple functions during the viral life cycle. These roles of Z involve interactions with viral and cellular proteins that remain incompletely understood. In this regard, Z inhibits viral RNA transcription and replication through direct interaction with the viral L polymerase. Here, we defined the L-binding domain of Tacaribe virus (TCRV) Z protein and the structural requirements mediating Z homo-oligomerization. By using site-directed mutagenesis, coimmunoprecipitation, and functional assays, we showed that residues R37, N39, W44, L50, and Y57, located around the zinc coordination site I, play a critical role in the Z-L interaction. We also found that Z protein from either TCRV or the pathogenic Junin virus (JUNV) self-associates into oligomeric forms in mammalian cells. Importantly, mutation of the myristoylation site, the strictly conserved residue G at position 2, severely impaired the ability of both TCRV Z and JUNV Z to self-interact as well as their capacity to accumulate at the plasma membrane, strongly suggesting that Z homo-oligomerization is associated with its myristoylation and cell membrane targeting. In contrast, disruption of the RING structure or substitution of W44 or N39, which are critical for L protein recognition, did not affect Z self-binding. Overall, the data presented here indicate that homo-oligomerization is not a requirement for Z-L interaction or Z-mediated polymerase activity inhibition.<\/jats:p>","DOI":"10.1128\/jvi.05691-11","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T02:09:58Z","timestamp":1317262198000},"page":"12304-12314","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Molecular Determinants of Arenavirus Z Protein Homo-Oligomerization and L Polymerase Binding"],"prefix":"10.1128","volume":"85","author":[{"given":"Maria Eugenia","family":"Loureiro","sequence":"first","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximiliano","family":"Wilda","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesica M.","family":"Levingston Macleod","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"D'Antuono","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Foscaldi","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Marino","family":"Buslje","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, Avenida Patricias Argentinas 435, Buenos Aires 1405, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Lopez","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Animal (CEVAN), Instituto de Ciencia y Tecnolog\u00eda Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnolog\u00eda (CONICET), Saladillo 2468, Buenos Aires C1440FFX, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"36","article-title":"Image processing with Image J","volume":"11","author":"Abramoff M. 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