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An Fab fragment of a monospecific antibody, which binds to residues 39 to 55 of VP1, was utilized to locate the N termini of VP1 in native (160S) particles in this \u201cbreathing\u201d state. Fab and virus were mixed and imaged via cryogenic electron microscopy. The resulting reconstruction showed the capsid expands similarly to the irreversibly altered cell entry intermediate (135S) particle, but the N terminus of VP1 is located near the 2-fold axes, instead of the \u201cpropeller tip\u201d as in 135S particles.<\/jats:p>","DOI":"10.1128\/jvi.05990-11","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T02:57:44Z","timestamp":1331175464000},"page":"5959-5962","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Structure of the Fab-Labeled \u201cBreathing\u201d State of Native Poliovirus"],"prefix":"10.1128","volume":"86","author":[{"given":"Jun","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily Y.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merja","family":"Roivainen","sequence":"additional","affiliation":[{"name":"National Institute for Health and Welfare (THL), Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Filman","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Hogle","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Belnap","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.3.1342-1354.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.1.73"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.2.673"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.01.017"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7745-7755.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.11.6466-6473.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.10.7125-7131.1996"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.5.1934-1945.1990"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.1.79"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)88343-4"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2004.02.006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.160757"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.5.1083-1087.1993"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.18.8757-8761.2000"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0769-2617(88)80004-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0256"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(03)01005-2"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.10.4697-4702.1990"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00557-09"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6774"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.6.3965-3970.1994"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05013-11"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-4-727"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8908"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1427"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0076-6879(81)78136-9","volume-title":"Interferons","author":"Rueckert RR","year":"1981","unstructured":"RueckertRR PallanschMA. 1981. 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