{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:15:40Z","timestamp":1775866540746,"version":"3.50.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A dependence of poliovirus on an unorthodox translation initiation mode can be targeted selectively to drive viral protein synthesis and cytotoxicity in malignant cells. Transformed cells are naturally susceptible to poliovirus, due to widespread ectopic upregulation of the poliovirus receptor, Necl-5, in ectodermal\/neuroectodermal cancers. Viral tumor cell killing and the host immunologic response it engenders produce potent, lasting antineoplastic effects in animal tumor models. Clinical application of this principle depends on unequivocal demonstration of safety in primate models for paralytic poliomyelitis. We conducted extensive dose-range-finding, toxicity, biodistribution, shedding, and neutralizing antibody studies of the prototype oncolytic poliovirus recombinant, PVS-RIPO, after intrathalamic inoculation in<jats:named-content content-type=\"genus-species\">Macaca fascicularis<\/jats:named-content>. These studies suggest that intracerebral PVS-RIPO inoculation does not lead to viral propagation in the central nervous system (CNS), does not cause histopathological CNS lesions or neurological symptoms that can be attributed to the virus, is not associated with extraneural virus dissemination or replication and does not induce shedding of virus with stool. Intrathalamic PVS-RIPO inoculation induced neutralizing antibody responses against poliovirus serotype 1 in all animals studied.<\/jats:p>","DOI":"10.1128\/jvi.06427-11","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T03:17:40Z","timestamp":1323919060000},"page":"2750-2759","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Attenuation of Neurovirulence, Biodistribution, and Shedding of a Poliovirus:Rhinovirus Chimera after Intrathalamic Inoculation in Macaca fascicularis"],"prefix":"10.1128","volume":"86","author":[{"given":"Elena Y.","family":"Dobrikova","sequence":"first","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Goetz","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Walters","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah K.","family":"Lawson","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James O.","family":"Peggins","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology Branch, National Cancer Institute, Frederick, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Muszynski","sequence":"additional","affiliation":[{"name":"Biological Research Branch, National Cancer Institute, Frederick, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheryl","family":"Ruppel","sequence":"additional","affiliation":[{"name":"Biopharmaceutical Development Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karyol","family":"Poole","sequence":"additional","affiliation":[{"name":"Biopharmaceutical Development Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L.","family":"Giardina","sequence":"additional","affiliation":[{"name":"Biopharmaceutical Development Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric M.","family":"Vela","sequence":"additional","affiliation":[{"name":"Battelle, Columbus, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Estep","sequence":"additional","affiliation":[{"name":"Battelle, Columbus, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Gromeier","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.524-530.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.122.3159.105"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.81.3.255"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(83)90046-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.10.6281-6290.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802845"},{"key":"e_1_3_2_8_2","first-page":"47","article-title":"Passage of poliomyelitis virus through the intestinal tract","volume":"6","author":"Clark PF","year":"1932","unstructured":"ClarkPF SchindlerJ. 1932. Passage of poliomyelitis virus through the intestinal tract. J. Prev. Med. 6:47\u201358.","journal-title":"J. Prev. Med."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900153106"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.05589-11"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.184"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.7.3310-3321.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/314548a0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00321-07"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2010.145"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2370"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.958-964.1999"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6803"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1965010"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00350-9"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/66.3.198"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.8.2636-2643.1988"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.231"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.3.1302-1309.1989"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00695-4"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"277","DOI":"10.4049\/jimmunol.53.3.277","article-title":"The recovery of poliomyelitis virus from the stools of experimentally infected monkeys and chimpanzees","volume":"53","author":"Melnick JL","year":"1946","unstructured":"MelnickJL. 1946. The recovery of poliomyelitis virus from the stools of experimentally infected monkeys and chimpanzees. J. Immunol. 53:277\u2013290.","journal-title":"J. Immunol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1215\/S1152851703000577"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.7.3147-3156.2006"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00243-06"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/biol.1993.1096"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011710"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.2.375"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.19.5793"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.115-122.2003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/334320a0"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.1.270"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1960.03820010007003"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.123.3209.1151"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00448-10"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-4-73"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1890"},{"key":"e_1_3_2_42_2","first-page":"364","volume-title":"Vaccines","author":"Sutter RW","year":"1999","unstructured":"SutterRW CochiSL MelnickJL. 1999. Live attenuated poliovirus vaccines, p 364\u2013408. In PlotkinSA OrensteinWA (ed), Vaccines, 3rd ed. W. B. Saunders, Philadelphia, PA.","edition":"3"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2457"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3713"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1795410"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.27.120193.002033"},{"key":"e_1_3_2_47_2","first-page":"1","article-title":"Recommendations for the production and control of poliomyelitis vaccine (oral)","volume":"910","author":"World Health Organization","year":"2002","unstructured":"World Health Organization. 2002. Recommendations for the production and control of poliomyelitis vaccine (oral). World Health Organ. Tech. Rep. Ser. 910:1\u2013104.","journal-title":"World Health Organ. Tech. Rep. Ser."},{"key":"e_1_3_2_48_2","first-page":"30","article-title":"Requirements for poliomyelitis vaccine (oral)","volume":"800","author":"World Health Organization","year":"1990","unstructured":"World Health Organization. 1990. Requirements for poliomyelitis vaccine (oral). World Health Organ. Tech. Rep. Ser. 800:30\u201386.","journal-title":"World Health Organ. Tech. Rep. Ser."},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.09.020"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.06427-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.06427-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,11]],"date-time":"2023-06-11T21:11:36Z","timestamp":1686517896000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.06427-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1128\/JVI.06427-11"],"URL":"https:\/\/doi.org\/10.1128\/jvi.06427-11","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]},"assertion":[{"value":"2011-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}