{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:50:32Z","timestamp":1790895032936,"version":"4.1.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Lymphocytic choriomeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), the causative agent of Lassa fever (LF), have extensive strain diversity and significant variations in pathogenicity for humans and experimental animals. The WE strain of LCMV (LCMV-WE), but not the Armstrong (Arm) strain, induces a fatal LF-like disease in rhesus macaques. We also demonstrated that LASV infection of human macrophages and endothelial cells resulted in reduced levels of proinflammatory cytokines. Here we have shown that cells infected with LASV or with LCMV-WE suppressed Toll-like receptor 2 (TLR2)-dependent proinflammatory cytokine responses. The persisting isolate LCMV clone 13 (CL13) also failed to stimulate interleukin-6 (IL-6) in macrophages. In contrast, nonpathogenic Mopeia virus, which is a genetic relative of LASV and LCMV-Arm induced robust responses that were TLR2\/Mal dependent, required virus replication, and were enhanced by CD14. Superinfection experiments demonstrated that the WE strain of LCMV inhibited the Arm-mediated IL-8 response during the early stage of infection. In cells transfected with the NF-\u03baB\u2013luciferase reporter, infection with LCMV-Arm resulted in the induction of NF-\u03baB, but cells infected with LCMV-WE and CL13 did not. These results suggest that pathogenic arenaviruses suppress NF-\u03baB-mediated proinflammatory cytokine responses in infected cells.<\/jats:p>","DOI":"10.1128\/jvi.06508-11","type":"journal-article","created":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T00:16:40Z","timestamp":1335399400000},"page":"7216-7226","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Pathogenic Old World Arenaviruses Inhibit TLR2\/Mal-Dependent Proinflammatory Cytokines\n            <i>In Vitro<\/i>"],"prefix":"10.1128","volume":"86","author":[{"given":"Melissa W.","family":"Hayes","sequence":"first","affiliation":[{"name":"Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, USA"},{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Ricardo","family":"Carrion","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerritt","family":"Nunneley","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei E.","family":"Medvedev","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria S.","family":"Salvato","sequence":"additional","affiliation":[{"name":"Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor S.","family":"Lukashevich","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Medicine, and the Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, University of Louisville, Louisville, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.07.001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_3_2_4_2","first-page":"799","article-title":"Lymphocytic choriomeningitis virus transmitted through solid organ transplantation\u2014Massachusetts, 2008","volume":"57","author":"Anonymous","year":"2008","unstructured":"Anonymous. 2008. Lymphocytic choriomeningitis virus transmitted through solid organ transplantation\u2014Massachusetts, 2008. MMWR Morb. Mortal. Wkly. Rep. 57:799\u2013801.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.6.3162-3169.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.02.002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.5.2861"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01948-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1792"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1011998107"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030149"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21161"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000455"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2079"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00429-11"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02876-06"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05304-11"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008016724243"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00536-07"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.3.1154-1162.2002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000388"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa053240"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000836"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1142"},{"key":"e_1_3_2_25_2","first-page":"146","article-title":"Lysosomotropic agents inhibit the penetration of arenaviruses into a culture of BHK-21 and Vero cells","volume":"35","author":"Glushakova SE","year":"1990","unstructured":"GlushakovaSE . 1990. Lysosomotropic agents inhibit the penetration of arenaviruses into a culture of BHK-21 and Vero cells. Vopr. Virusol. 35:146\u2013150.","journal-title":"Vopr. Virusol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2007.10.002"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001137"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-205"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.2008\/004721-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.10452"},{"key":"e_1_3_2_31_2","first-page":"486","article-title":"Lymphocytic choriomeningitis virus. A neglected pathogen of man","volume":"116","author":"Jahrling PB","year":"1992","unstructured":"JahrlingPB PetersCJ. 1992. Lymphocytic choriomeningitis virus. A neglected pathogen of man. Arch. Pathol. Lab. Med. 116:486\u2013488.","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01640-07"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.02.042"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.22.14282-14296.2005"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90514-P"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.3.1727-1737.2003"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-004-0385-9"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2206"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199912)59:4<552::AID-JMV21>3.0.CO;2-A"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1086\/320722"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.2797"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00882-07"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00555-06"},{"key":"e_1_3_2_44_2","first-page":"75","article-title":"Lassa fever","volume":"262","author":"McCormick JB","year":"2002","unstructured":"McCormickJB Fisher-HochSP. 2002. Lassa fever. Curr. Top. Microbiol. Immunol. 262:75\u2013109.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198601023140104"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/35100529"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.142"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00671-08"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.11.023"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02120-10"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1009674"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.4.1863-1869.1991"},{"key":"e_1_3_2_53_2","first-page":"715","volume-title":"Virus taxonomy, classification and nomenclature of viruses.","author":"Salvato MS","year":"2012","unstructured":"SalvatoMS . 2012. Family Arenaviridae, p 715\u2013723. In KingAMQ AdamsMJ CarstensEB LefkowitzEJ (ed), Virus taxonomy, classification and nomenclature of viruses. Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Inc, San Diego, CA."},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.5.3113"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06451-11"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.1.284.284"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019304108"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.01.002"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2010.07.018"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-010-8162-1"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06284-11"},{"key":"e_1_3_2_62_2","first-page":"291","volume-title":"Abstr. 29th Am. Soc. Virol. Annu. Meet.","author":"Zapata J","year":"2010","unstructured":"ZapataJ . 2010. Expression of coagulation factor thrombomodulin is increased in cells exposed to Lassa virus, p 291. Abstr. 29th Am. Soc. Virol. Annu. Meet., Bozeman, MT."},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2011.07.015"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00155-10"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01770-08"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425730"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.06508-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.06508-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:11:14Z","timestamp":1646493074000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.06508-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":65,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1128\/JVI.06508-11"],"URL":"https:\/\/doi.org\/10.1128\/jvi.06508-11","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7]]},"assertion":[{"value":"2011-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}