{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:13:25Z","timestamp":1792588405737,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We previously reported that mice lacking alpha\/beta and gamma interferon receptors (IFN-\u03b1\/\u03b2R and -\u03b3R) uniformly exhibit paralysis following infection with the dengue virus (DENV) clinical isolate PL046, while only a subset of mice lacking the IFN-\u03b3R alone and virtually no mice lacking the IFN-\u03b1\/\u03b2R alone develop paralysis. Here, using a mouse-passaged variant of PL046, strain S221, we show that in the absence of the IFN-\u03b1\/\u03b2R, signaling through the IFN-\u03b3R confers approximately 140-fold greater resistance against systemic vascular leakage-associated dengue disease and virtually complete protection from dengue-induced paralysis. Viral replication in the spleen was assessed by immunohistochemistry and flow cytometry, which revealed a reduction in the number of infected cells due to IFN-\u03b3R signaling by 2 days after infection, coincident with elevated levels of IFN-\u03b3 in the spleen and serum. By 4 days after infection, IFN-\u03b3R signaling was found to restrict DENV replication systemically. Clearance of DENV, on the other hand, occurred in the absence of IFN-\u03b3R, except in the central nervous system (CNS) (brain and spinal cord), where clearance relied on IFN-\u03b3 from CD8\n            <jats:sup>+<\/jats:sup>\n            T cells. These results demonstrate the roles of IFN-\u03b3R signaling in protection from initial systemic and subsequent CNS disease following DENV infection and demonstrate the importance of CD8\n            <jats:sup>+<\/jats:sup>\n            T cells in preventing DENV-induced CNS disease.\n          <\/jats:p>","DOI":"10.1128\/jvi.06743-11","type":"journal-article","created":{"date-parts":[[2012,9,13]],"date-time":"2012-09-13T00:37:54Z","timestamp":1347496674000},"page":"12561-12570","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Gamma Interferon (IFN-\u03b3) Receptor Restricts Systemic Dengue Virus Replication and Prevents Paralysis in IFN-\u03b1\/\u03b2 Receptor-Deficient Mice"],"prefix":"10.1128","volume":"86","author":[{"given":"Tyler R.","family":"Prestwood","sequence":"first","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malika M.","family":"Morar","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00ebl M.","family":"Zellweger","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn","family":"Miller","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica M.","family":"May","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.","family":"Yauch","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Lada","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujan","family":"Shresta","sequence":"additional","affiliation":[{"name":"Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-003-0785-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1054\/jocn.2000.0735"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.10.007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2003.tb00646.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000790"},{"key":"e_1_3_2_7_2","first-page":"107","article-title":"Immunohistochemical characterization of a new monoclonal antibody reactive with dengue virus-infected cells in frozen tissue using immunoperoxidase technique","volume":"14","author":"Bhoopat L","year":"1996","unstructured":"BhoopatL. 1996. 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