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We generated monoclonal antibodies (MAbs) against Jurkat T cells and selected several IgM MAbs that strongly inhibited HTLV-1 but not human immune deficiency virus type 1 (HIV-1) cell-to-cell infection. These MAbs recognized the so-called Tn antigen (GalNAc\u03b11-<jats:italic>O<\/jats:italic>-Ser\/Thr) that arises on Jurkat cells from a mutation in the T-synthase-specific chaperone Cosmc and the consequent loss of<jats:italic>O<\/jats:italic>-glycan elongation. Anti-Tn MAbs precipitated two major<jats:italic>O<\/jats:italic>-glycan carrier proteins, CD43 and CD45, and caused a strong aggregation of Jurkat cells. The restoration of O-glycosylation in Jurkat cells by stably transducing the wild-type<jats:italic>Cosmc<\/jats:italic>gene resulted in a 3- to 4-fold increase in the level of surface expression of CD43 and enhanced HTLV-1 transmission 10-fold in comparison to that of parental cells. The short hairpin RNA (shRNA) knockdown of CD43 or CD45 expression in Jurkat-Cosmc, HBP-ALL, and CEM T cells decreased HTLV-1 infection severalfold. The knockdown of CD45 in Jurkat cells severely reduced both HTLV-1 and HIV-1 infections, but Cosmc coexpression partially rescued infection. HTLV-1 proteins, which assembled in small patches on Jurkat cells, formed large clusters on the surface of Jurkat-Cosmc cells. These data indicate that large aggregates of HTLV-1 assemblies are more infectious than multiple clustered virions. We suggest that heavily O-glycosylated CD43 and CD45 molecules render cells less adhesive, prevent inappropriate cell-cell contacts, and favor the assembly of HTLV-1 particles into large, highly infectious structures on the surface of T cells.<\/jats:p>","DOI":"10.1128\/jvi.06993-11","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T03:17:40Z","timestamp":1323919060000},"page":"2447-2458","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Role of O-Glycosylation and Expression of CD43 and CD45 on the Surfaces of Effector T Cells in Human T Cell Leukemia Virus Type 1 Cell-to-Cell Infection"],"prefix":"10.1128","volume":"86","author":[{"given":"Dmitriy","family":"Mazurov","sequence":"first","affiliation":[{"name":"Laboratory of Immunochemistry, Institute of Immunology, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ilinskaya","sequence":"additional","affiliation":[{"name":"HIV Drug Resistance Program, NCI-Frederick, Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela","family":"Heidecker","sequence":"additional","affiliation":[{"name":"HIV Drug Resistance Program, NCI-Frederick, Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Filatov","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry, Institute of Immunology, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.53.8.581"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.3792"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(99)00069-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwr178"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23493"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00797.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00381-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb08022.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00231-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.18.8461-8468.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2008.46"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.13.6173-6182.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.4.905-910.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2006.09.014"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08485.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-7-99"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"2250","DOI":"10.4049\/jimmunol.159.5.2250","article-title":"CD99 (MIC2) regulates the LFA-1\/ICAM-1-mediated adhesion of lymphocytes, and its gene encodes both positive and negative regulators of cellular adhesion","volume":"159","author":"Hahn JH","year":"1997","unstructured":"HahnJH. 1997. 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