{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:17:23Z","timestamp":1758709043910},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1969,4,1]],"date-time":"1969-04-01T00:00:00Z","timestamp":-23760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1969,4]]},"abstract":"<jats:p>\n            Infection of baby hamster kidney cells (BHK-21\/13) with Saint Louis encephalitis (SLE) virus depressed the rate of protein and ribonucleic acid (RNA) synthesis until viral RNA synthesis began 6 hr postinfection (PI). Virus-directed RNA synthesis was subsequently inhibited until 12 hr PI when virion maturation began. The rate of protein synthesis reached a peak 6 hr PI and was subsequently depressed until just before the onset of virion maturation. Density gradient analysis of phenol-extracted RNA from actinomycin-treated infected cells indicated that, at 6 to 8 hr and again at 12 to 20 hr PI, three species of viral-specific RNA were synthesized. The most rapid sedimenting form (43\n            <jats:italic>S<\/jats:italic>\n            ) was ribonuclease-sensitive and had a base composition similar to the RNA isolated from mature virions. The 20\n            <jats:italic>S<\/jats:italic>\n            RNA species was ribonuclease-resistant and had a sedimentation coefficient and base composition similar to the replicative form associated with other arbovirus infections. The 26\n            <jats:italic>S<\/jats:italic>\n            RNA was ribonuclease-resistant (0.2 \u03bcg\/ml, 0.1\n            <jats:sc>m<\/jats:sc>\n            NaCl, 25 C, 30 min) and had a nucleotide base composition closer to the 20\n            <jats:italic>S<\/jats:italic>\n            form than to the values for 43\n            <jats:italic>S<\/jats:italic>\n            RNA. Five-minute pulse labeling of infected cultures during the period viral RNA synthesis was maximal resulted in labeling of only the 20S to 22S RNA fractions. With pulse-labeling periods of 10 min, both the 20\n            <jats:italic>S<\/jats:italic>\n            and 26\n            <jats:italic>S<\/jats:italic>\n            RNA species were radioactive. Periods of radioactive labeling of as long as 15 min were required before the 43\n            <jats:italic>S<\/jats:italic>\n            form was radioactively labeled. These results suggest that the 20\n            <jats:italic>S<\/jats:italic>\n            and 26\n            <jats:italic>S<\/jats:italic>\n            RNA may be intermediate forms in the synthesis of 43\n            <jats:italic>S<\/jats:italic>\n            viral RNA.\n          <\/jats:p>","DOI":"10.1128\/jvi.3.4.385-394.1969","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:59:03Z","timestamp":1578272343000},"page":"385-394","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Synthesis of Saint Louis Encephalitis Virus Ribonucleic Acid in BHK-21\/13 Cells"],"prefix":"10.1128","volume":"3","author":[{"given":"Dennis W.","family":"Trent","sequence":"first","affiliation":[{"name":"Department of Microbiology, Brigham Young University, Provo, Utah 84601"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie C.","family":"Swensen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Brigham Young University, Provo, Utah 84601"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ateef A.","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Brigham Young University, Provo, Utah 84601"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.3.4.385-394.1969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.3.4.385-394.1969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:57:21Z","timestamp":1646495841000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.3.4.385-394.1969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1969,4]]}},"alternative-id":["10.1128\/jvi.3.4.385-394.1969"],"URL":"https:\/\/doi.org\/10.1128\/jvi.3.4.385-394.1969","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1969,4]]},"assertion":[{"value":"1969-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}