{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T19:03:36Z","timestamp":1700507016115},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1979,6,1]],"date-time":"1979-06-01T00:00:00Z","timestamp":297043200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1979,6]]},"abstract":"<jats:p>A temperature sensitive mutant of vesicular stomatitis virus which does not mature properly when grown at 39\u00b0C promoted extensive fusion of murine neuroblastoma cells at this nonpermissive temperature. Polykaryocytes apparently formed as a result of fusion from within the cells that requires low doses of infectious virions for its promotion and is dependent on viral protein synthesis. Although 90% of infected N-18 neuroblastoma cells were fused by 15 h after infection, larger polykaryocytes continued to form, leading to an average of 28 nuclei per polykaryocyte as a result of polykaryocytes fusing to each other. Two neuroblastoma cell lines have been observed to undergo fusion, whereas three other cell lines (BHK-21, CHO, and 3T3) were incapable of forming polykaryocytes, suggesting that nervous system-derived cells are particularly susceptible to vesicular stomatitis virus-induced fusion. Although the normal assembly of the protein components of this virus is deficient at 39\u00b0C, the G glycoprotein was inserted into the infected cell membranes at this temperature. Two lines of evidence suggest that the expression of G at the cell surface promotes this polykaryocyte formation: (i) inhibition of glycosylation, which may be involved in the migration of the G protein to the cellular plasma membranes, will inhibit the cell fusion reaction; (ii) addition of antiserum, directed toward the purified G glycoprotein, will also inhibit cell fusion.<\/jats:p>","DOI":"10.1128\/jvi.30.3.883-890.1979","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:48:27Z","timestamp":1578271707000},"page":"883-890","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Neuroblastoma Cell Fusion by a Temperature-Sensitive Mutant of Vesicular Stomatitis Virus"],"prefix":"10.1128","volume":"30","author":[{"given":"Joseph V.","family":"Hughes","sequence":"first","affiliation":[{"name":"Division of Biology, Kansas State University, Manhattan Kansas 66506"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J.","family":"Dille","sequence":"additional","affiliation":[{"name":"Division of Biology, Kansas State University, Manhattan Kansas 66506"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta L.","family":"Thimmig","sequence":"additional","affiliation":[{"name":"Division of Biology, Kansas State University, Manhattan Kansas 66506"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry C.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Division of Biology, Kansas State University, Manhattan Kansas 66506"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley G.","family":"Rabinowitz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Northwestern University Medical School and Veterans Administration Lakeside Hospital, Chicago, Illinois 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro C.","family":"Dal Canto","sequence":"additional","affiliation":[{"name":"Department of Pathology (Neuropathology), Northwestern University Medical School and Veterans Administration Lakeside Hospital, Chicago, Illinois 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.30.3.883-890.1979","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.30.3.883-890.1979","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T16:05:24Z","timestamp":1646496324000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.30.3.883-890.1979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,6]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1979,6]]}},"alternative-id":["10.1128\/jvi.30.3.883-890.1979"],"URL":"https:\/\/doi.org\/10.1128\/jvi.30.3.883-890.1979","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,6]]},"assertion":[{"value":"1979-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}