{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:15:42Z","timestamp":1790766942411,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1996,2,1]],"date-time":"1996-02-01T00:00:00Z","timestamp":823132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1996,2]]},"abstract":"<jats:p>Herpes simplex virus type 1 (HSV-1) infection of the murine cornea induces the rapid infiltration of neutrophils. We investigated whether these cells could influence virus replication. BALB\/c mice treated with monoclonal antibody (MAb) RB6-8C5 experienced a profound depletion of neutrophils in the bloodstream, spleen, and cornea. In these animals, virus titers in the eye were significantly higher than those in the immunoglobulin G-treated controls at 3 days postinfection. By day 9, virus was no longer detectable in the controls, whereas titers of 10(3) to 10(6) PFU were still present in the neutrophil-depleted hosts. Furthermore, virus spread more readily to the skin and brains of MAb RB6-8C5-treated animals, rendering them significantly more susceptible to HSV-1-induced blepharitis and encephalitis. Only 25% of the treated animals survived, whereas all of the controls lived. Although MAb RB6-8C5 treatment did not alter the CD4+ T-cell, B-cell, natural killer cell, or macrophage populations, the CD8+ T-cell population was partially reduced. Therefore, the experiments were repeated in severe combined immunodeficiency mice, which lack CD8+ T cells. Again virus growth was found to be significantly elevated in the eyes, trigeminal ganglia, and brains of the MAb RB6-8C5-treated hosts. These results strongly indicate that in both immunocompetent and immunodeficient mice, neutrophils play a significant role in helping to control the replication and spread of HSV-1 after corneal infection.<\/jats:p>","DOI":"10.1128\/jvi.70.2.898-904.1996","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:44:10Z","timestamp":1578347050000},"page":"898-904","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":164,"title":["Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea"],"prefix":"10.1128","volume":"70","author":[{"given":"T M","family":"Tumpey","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S H","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J E","family":"Oakes","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R N","family":"Lausch","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.70.2.898-904.1996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.70.2.898-904.1996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:15:32Z","timestamp":1646486132000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.70.2.898-904.1996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,2]]}},"alternative-id":["10.1128\/jvi.70.2.898-904.1996"],"URL":"https:\/\/doi.org\/10.1128\/jvi.70.2.898-904.1996","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2]]},"assertion":[{"value":"1996-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}