{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:22:15Z","timestamp":1791264135604,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1997,1,1]],"date-time":"1997-01-01T00:00:00Z","timestamp":852076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>An Epstein-Barr virus transcript (designated D-HIT [Daudi high-level-inducible transcript]), constitutively expressed at low levels in the Burkitt's lymphoma (BL)-derived cell line Daudi, can be induced with tetradecanoylphorbol acetate or n-butyrate or, in combination, to about 1% of the levels of high-molecular-weight RNAs in cells. The transcript can also be induced in some other EBV-positive BL-derived cells but to a much lesser extent, particularly in lines that can give rise to productive infection. D-HIT is viral in origin and is composed largely of repetitive sequence. It is polyadenylated but mainly nuclear in location and is highly structured, sensitive only to double-strand-specific RNase. It is endogenously expressed in interferon-sensitive Daudi strains but not in an insensitive strain, Daudi 100K. D-HIT contains a part of a viral open reading frame (designated LF3, and deleted in the prototype B95-8 strain), using an internal polyadenylation (AAUAAA) sequence as a signal to specify processing of its 3' end. In Daudi cells, the promoter contains a putative hinge structure, as found in some interferon-inducible genes and c-myc. Since D-HIT lies adjacent to, probably even encompassing, one of the two viral lytic origins (D(R)) of replication, it may have a role in the regulation of DNA replication. Alternatively, or in addition via its double-stranded structure, D-HIT may play a regulatory role in interferon pathways. Its promoter could be of value for studying expression in constructions containing heterologous genes.<\/jats:p>","DOI":"10.1128\/jvi.71.1.84-94.1997","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:52:44Z","timestamp":1578347564000},"page":"84-94","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the Burkitt's lymphoma line Daudi"],"prefix":"10.1128","volume":"71","author":[{"given":"Y","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Virology, Royal Postgraduate Medical School, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P R","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Virology, Royal Postgraduate Medical School, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Karran","sequence":"additional","affiliation":[{"name":"Department of Virology, Royal Postgraduate Medical School, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q L","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Virology, Royal Postgraduate Medical School, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B E","family":"Griffin","sequence":"additional","affiliation":[{"name":"Department of Virology, Royal Postgraduate Medical School, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.71.1.84-94.1997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.71.1.84-94.1997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:25:45Z","timestamp":1646486745000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.71.1.84-94.1997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1128\/jvi.71.1.84-94.1997"],"URL":"https:\/\/doi.org\/10.1128\/jvi.71.1.84-94.1997","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]},"assertion":[{"value":"1997-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}