{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T21:18:31Z","timestamp":1785187111290,"version":"3.55.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We previously reported that the region corresponding to amino acids 197 to 216 of the gp46 surface glycoprotein (gp46-197) served as a binding domain for the interaction between gp46 and trypsin-sensitive membrane components of the target cell, leading to syncytium formation induced by human T-cell lymphotropic virus type 1 (HTLV-1)-bearing cells. Our new evidence shows that the 71-kDa heat shock cognate protein (HSC70) acts as a cellular receptor for syncytium formation. Using affinity chromatography with the peptide gp46-197, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we isolated three components (bands A, B, and C) from MOLT-4 cell lysate which exhibited specific interactions with gp46 and inhibitory activities for syncytium formation induced by HTLV-1-bearing cells. Band A and B components were identified as HSC70 and \u03b2-actin, respectively, through amino acid sequencing by tandem mass spectrometry and immunostaining with specific monoclonal antibodies. Band C is likely to be a nonprotein component, because full activity for syncytium formation was seen after extensive trypsin digestion. Anti-HSC70 monoclonal antibody clearly blocked syncytium formation in a coculture of HTLV-1-bearing cells and indicator cells, whereas no inhibition was seen with anti-\u03b2-actin monoclonal antibody. Furthermore, flow cytometric analysis indicated that anti-HSC70 antibody reacted with MOLT-4 cells. Thus, we propose that HSC70 expressed on the target cell surface acts as a cellular acceptor to gp46 exposed on the HTLV-1-infected cell for syncytium formation, thereby leading to cell-to-cell transmission of HTLV-1.<\/jats:p>","DOI":"10.1128\/jvi.72.1.535-541.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T18:00:39Z","timestamp":1577815239000},"page":"535-541","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["71-Kilodalton Heat Shock Cognate Protein Acts as a Cellular Receptor for Syncytium Formation Induced by Human T-Cell Lymphotropic Virus Type 1"],"prefix":"10.1128","volume":"72","author":[{"given":"Yasuko","family":"Sagara","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Fukuoka Red Cross Blood Center, Fukuoka 818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuzo","family":"Ishida","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Fukuoka Red Cross Blood Center, Fukuoka 818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Inoue","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Fukuoka Red Cross Blood Center, Fukuoka 818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Shiraki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Fukuoka Red Cross Blood Center, Fukuoka 818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Maeda","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Fukuoka Red Cross Blood Center, Fukuoka 818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.1.485-493.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198805053181801"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/305502a0"},{"key":"e_1_3_2_5_2","first-page":"844","article-title":"Infectivity dissociated from transforming activity in a human retrovirus, adult T-cell leukemia virus","volume":"73","author":"Chosa T.","year":"1982","unstructured":"Chosa T. 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