{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:05:43Z","timestamp":1791234343467,"version":"4.1.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Progression of human immunodeficiency virus (HIV) disease is associated with massive death of CD4<jats:sup>+<\/jats:sup>T cells along with death and\/or dysfunction of CD8<jats:sup>+<\/jats:sup>T cells. In vivo, both HIV infection per se and host factors may contribute to the death and\/or dysfunction of CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cells. Progression of HIV disease is often characterized by a switch from R5 to X4 HIV type 1 (HIV-1) variants. In human lymphoid tissues ex vivo, it was shown that HIV infection is sufficient for CD4<jats:sup>+<\/jats:sup>T-cell depletion. Here we address the question of whether infection of human lymphoid tissue ex vivo with prototypic R5 or X4 HIV variants also depletes or impairs CD8<jats:sup>+<\/jats:sup>T cells. We report that whereas productive infection of lymphoid tissue ex vivo with R5 and X4 HIV-1 isolates induced apoptosis in CD4<jats:sup>+<\/jats:sup>T cells, neither viral isolate induced apoptosis in CD8<jats:sup>+<\/jats:sup>T cells. Moreover, in both infected and control tissues we found similar numbers of CD8<jats:sup>+<\/jats:sup>T cells and similar production of cytokines by these cells in response to phorbol myristate acetate or anti-CD3\u2013anti-CD28 stimulation. Thus, whereas HIV-1 infection per se in human lymphoid tissue is sufficient to trigger apoptosis in CD4<jats:sup>+<\/jats:sup>T cells, the death of CD8<jats:sup>+<\/jats:sup>T cells apparently requires additional factors.<\/jats:p>","DOI":"10.1128\/jvi.74.17.8077-8084.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"8077-8084","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Human Immunodeficiency Virus Type 1 Induces Apoptosis in CD4<sup>+<\/sup>but Not in CD8<sup>+<\/sup>T Cells in Ex Vivo-Infected Human Lymphoid Tissue"],"prefix":"10.1128","volume":"74","author":[{"given":"Jean-Charles","family":"Grivel","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Malkevitch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid","family":"Margolis","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1542","article-title":"Presence of CD3+CD8+Bcl-2(low) lymphocytes undergoing apoptosis and activated macrophages in lymph nodes of HIV-1+ patients","volume":"146","author":"Bofill M.","year":"1995","unstructured":"Bofill M. Gombert W. Borthwick N. J. Akbar A. N. McLaughlin J. E. Lee C. A. Johnson M. A. Pinching A. J. Janossy G. Presence of CD3+CD8+Bcl-2(low) lymphocytes undergoing apoptosis and activated macrophages in lymph nodes of HIV-1+ patients.Am. J. Pathol. 146 1995 1542 1555","journal-title":"Am. J. 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Ashwell. Exploiting the apoptotic process for management of HIV: are we there yet? Apoptosis in press."},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5443.1353"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.74.17.8077-8084.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.74.17.8077-8084.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T15:02:44Z","timestamp":1682262164000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.74.17.8077-8084.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":69,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1128\/JVI.74.17.8077-8084.2000"],"URL":"https:\/\/doi.org\/10.1128\/jvi.74.17.8077-8084.2000","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]},"assertion":[{"value":"2000-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-06-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}