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Live-attenuated YF vaccine is one of the most effective viral vaccines available today. Important advantages include its ability to induce long-lasting immunity, its safety, its affordability, and its documented efficacy. In this study, recombinant live-attenuated (strain 17D) YF viruses were constructed to express a cytotoxic T-lymphocyte epitope derived from chicken ovalbumin (SIINFEKL). These recombinant viruses replicated comparably to the 17D vaccine strain in cell culture and stably expressed the ovalbumin antigen, and infected cells presented the antigen in the context of major histocompatibility complex class I. Inoculation of mice with recombinant YF virus elicited SIINFEKL-specific CD8<jats:sup>+<\/jats:sup>lymphocytes and induced protective immunity against challenge with lethal doses of malignant melanoma cells expressing ovalbumin. Furthermore, active immunotherapy with recombinant YF viruses induced regression of established solid tumors and pulmonary metastases. Thus, recombinant YF viruses are attractive viral vaccine vector candidates for the development of therapeutic anticancer vaccines.<\/jats:p>","DOI":"10.1128\/jvi.74.19.9197-9205.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"9197-9205","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Recombinant Yellow Fever Viruses Are Effective Therapeutic Vaccines for Treatment of Murine Experimental Solid Tumors and Pulmonary Metastases"],"prefix":"10.1128","volume":"74","author":[{"given":"Andre\u0301s","family":"McAllister","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of California, San Francisco, California 94143-0414"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra E.","family":"Arbetman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of California, San Francisco, California 94143-0414"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Mandl","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of California, San Francisco, California 94143-0414"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Pen\u0303a-Rossi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of California, San Francisco, California 94143-0414"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul","family":"Andino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of California, San Francisco, California 94143-0414"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"S57","article-title":"Dicistronic polioviruses as expression vectors for foreign genes","volume":"10","author":"Alexander L.","year":"1994","unstructured":"Alexander L. 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