{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:07:29Z","timestamp":1791418049501,"version":"4.3.3"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The human polyomavirus JC virus (JCV) is the etiologic agent of a fatal central nervous system (CNS) demyelinating disease known as progressive multifocal leukoencephalopathy (PML). PML occurs predominantly in immunosuppressed patients and has increased dramatically as a result of the AIDS pandemic. The major target cell of JCV infection and lytic replication in the CNS is the oligodendrocyte. The mechanisms by which JCV initiates and establishes infection of these glial cells are not understood. The initial interaction between JCV and glial cells involves virus binding to N-linked glycoproteins containing terminal \u03b1(2-6)-linked sialic acids. The subsequent steps of entry and targeting of the viral genome to the nucleus have not been described. In this report, we compare the kinetics and mechanisms of infectious entry of JCV into human glial cells with that of the related polyomavirus, simian virus 40 (SV40). We demonstrate that JCV, unlike SV40, enters glial cells by receptor-mediated clathrin-dependent endocytosis.<\/jats:p>","DOI":"10.1128\/jvi.74.5.2288-2292.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:06:37Z","timestamp":1027749997000},"page":"2288-2292","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":220,"title":["JC Virus Enters Human Glial Cells by Clathrin-Dependent Receptor-Mediated Endocytosis"],"prefix":"10.1128","volume":"74","author":[{"given":"M. T.","family":"Pho","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02912"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ashok","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02912"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J.","family":"Atwood","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02912"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.7.11.1825"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-6-1469"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10909"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90909-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.10.4474-4477.1989"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/13550289509111009"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.6.1.78-86.1970"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.4.2037-2045.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4301"},{"key":"e_1_3_2_11_2","unstructured":"Harlow\nE.\nLane\nD.\nAntibodies: a laboratory manual.\n1988\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35701-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.90.3.819-821.1965"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.3.595"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809113481"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.6.4643-4649.1998"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.19.2.620-636.1976"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.1.49"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.4.1257"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.28.3.936-944.1978"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.10.7004-7012.1996"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1247\/csf.11.135"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.6.2931"},{"key":"e_1_3_2_24_2","unstructured":"Shah\nK. V.\nPolyomaviruses\nFields virology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP. M.\n1996\n2027\n2043\nLippincott-Raven\nPhiladelphia Pa"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.1.47"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90425-X"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","unstructured":"Walker\nD. L.\nFrisque\nR. J.\nThe biology and molecular biology of JC virus\nThe Papovaviridae.\nSalzman\nN. P.\n1986\n327\n377\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4613-2221-4_6"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.5.1107"},{"key":"e_1_3_2_29_2","unstructured":"Wei G. C. K. Liu and W. J. Atwood. JC virus binds to primary human glial cells tonsillar stromal cells and B-lymphocytes but not to T-lymphocytes. J. Neurovirol. in press."}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.74.5.2288-2292.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.74.5.2288-2292.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:34:39Z","timestamp":1646440479000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.74.5.2288-2292.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1128\/JVI.74.5.2288-2292.2000"],"URL":"https:\/\/doi.org\/10.1128\/jvi.74.5.2288-2292.2000","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]},"assertion":[{"value":"1999-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-12-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}