{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T19:30:16Z","timestamp":1793734216883,"version":"4.6.1"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2000,4,15]],"date-time":"2000-04-15T00:00:00Z","timestamp":955756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2000,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genome of lymphocytic choriomeningitis virus (LCMV) consists of two negative-sense single-stranded RNA segments, designated L and S. Both segments contain two viral genes in an ambisense coding strategy, with the genes being separated by an intergenic region (IGR). We have developed a reverse genetic system that allows the investigation of\n            <jats:italic>cis<\/jats:italic>\n            -acting signals and\n            <jats:italic>trans<\/jats:italic>\n            -acting factors involved in transcription and replication of LCMV. To this end, we constructed an LCMV S minigenome consisting of a negative-sense copy of the chloramphenicol acetyltransferase (CAT) reporter gene flanked upstream by the S 5\u2032 untranslated region (UTR) and IGR and downstream by the S 3\u2032 UTR. CAT expression was detected in LCMV-infected cells transfected with the minigenome RNA. Intracellular coexpression of the LCMV minigenome and LCMV L and NP proteins supplied from cotransfected plasmids driven by the T7 RNA polymerase provided by the recombinant vaccinia virus vTF7-3 resulted in high levels of CAT activity and synthesis of subgenomic CAT mRNA and antiminigenome RNA species. Thus, L and NP represent the minimal viral\n            <jats:italic>trans<\/jats:italic>\n            -acting factors required for efficient RNA synthesis mediated by LCMV polymerase.\n          <\/jats:p>","DOI":"10.1128\/jvi.74.8.3470-3477.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:37Z","timestamp":1027764397000},"page":"3470-3477","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":222,"title":["NP and L Proteins of Lymphocytic Choriomeningitis Virus (LCMV) Are Sufficient for Efficient Transcription and Replication of LCMV Genomic RNA Analogs"],"prefix":"10.1128","volume":"74","author":[{"given":"Ki Jeong","family":"Lee","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel S.","family":"Novella","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael N.","family":"Teng","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B. 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