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Lentiviruses, including human immunodeficiency virus type 1 (HIV-1), encode Env glycoproteins with unusually long cytoplasmic tails, the functions of which have not been fully elucidated. In this study, we examine the effects on virus replication of a number of mutations in a helical motif (\u03b1-helix 2) located near the center of the HIV-1 gp41 cytoplasmic tail. We find that, in T-cell lines, small deletions in this domain disrupt the incorporation of Env glycoproteins into virions and markedly impair virus infectivity. Through the analysis of viral revertants, we demonstrate that a single amino acid change (34VI) in the matrix domain of Gag reverses the Env incorporation and infectivity defect imposed by a small deletion near the C terminus of \u03b1-helix 2. These results provide genetic evidence, in the context of infected T cells, for an interaction between HIV-1 matrix and the gp41 cytoplasmic tail and identify domains of both proteins involved in this putative interaction.<\/jats:p>","DOI":"10.1128\/jvi.74.8.3548-3554.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:06:37Z","timestamp":1027749997000},"page":"3548-3554","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":206,"title":["Genetic Evidence for an Interaction between Human Immunodeficiency Virus Type 1 Matrix and \u03b1-Helix 2 of the gp41 Cytoplasmic Tail"],"prefix":"10.1128","volume":"74","author":[{"given":"Tsutomu","family":"Murakami","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0460"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric O.","family":"Freed","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0460"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.59.2.284-291.1986"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1997.13.665"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.25.15773"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00964.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1689-1696.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.11.6616-6625.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.4.2674-2684.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360290122"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.1.341-351.1996"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.4.2503-2512.1994"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.41.23883"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.3.1984-1989.1995"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.6.3306-3315.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90341-3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.7.3099"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7555"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.11.6810-6818.1995"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.5.4116-4126.1998"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.4.2232-2239.1992"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi962935r"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)54085-X"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.1.162-169.1991"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3289117"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90685-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.8.4857-4861.1994"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2305256"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.6.3824-3830.1995"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1457"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1991.7.511"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7659-7663.1998"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.1.343"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.8.1389"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8839"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/378743a0"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.9.2699"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"473","DOI":"10.4049\/jimmunol.155.1.473","article-title":"Endocytosis of endogenously synthesized HIV-1 envelope protein. 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