{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T04:54:18Z","timestamp":1794545658808,"version":"4.9.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    \u03b1-Dystroglycan (\u03b1-DG) was recently identified as a receptor for lymphocytic choriomeningitis virus (LCMV) and several other arenaviruses, including Lassa fever virus (W. Cao, M. D. Henry, P. Borrow, H. Yamada, J. H. Elder, E. V. Ravkov, S. T. Nichol, R. W. Compans, K. P. Campbell, and M. B. A. Oldstone, Science 282:2079\u20132081, 1998). Data presented in this paper indicate that the affinity of binding of LCMV to \u03b1-DG determines viral tropism and the outcome of infection in mice. To characterize this relationship, we evaluated the interaction between \u03b1-DG and several LCMV strains, variants, and reassortants. These viruses could be divided into two groups with respect to affinity of binding to \u03b1-DG, dependence on this protein for cell entry, viral tropism, and disease course. Viruses that exhibited high-affinity binding to \u03b1-DG displayed a marked dependence on \u03b1-DG for cell entry and were blocked from infecting mouse 3T6 fibroblasts by 1 to 4 nM soluble \u03b1-DG. In addition, high-affinity binding to \u03b1-DG correlated with an ability to infiltrate the white pulp (T-dependent) area of the spleen, cause ablation of the cytotoxic T-lymphocyte (CTL) response by day 7 postinfection, and establish a persistent infection. In contrast, viruses with a lower affinity of binding to \u03b1-DG were only partially inhibited from infecting \u03b1-DG\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    embryonic stem cells and required a concentration of soluble \u03b1-DG higher than 100 nM to prevent infection of mouse 3T6 fibroblasts. These viruses that bound at low affinity were mainly restricted to the splenic red pulp, and the host generated an effective CTL response that rapidly cleared the infection. Reassortants of viruses that bound to \u03b1-DG at high and low affinities were used to map genes responsible for the differences described to the S RNA, containing the virus attachment protein glycoprotein 1.\n                  <\/jats:p>","DOI":"10.1128\/jvi.75.1.448-457.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:06:37Z","timestamp":1027749997000},"page":"448-457","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":139,"title":["Differences in Affinity of Binding of Lymphocytic Choriomeningitis Virus Strains to the Cellular Receptor \u03b1-Dystroglycan Correlate with Viral Tropism and Disease Kinetics"],"prefix":"10.1128","volume":"75","author":[{"given":"Sara C.","family":"Smelt","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Persephone","family":"Borrow","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->The Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, United Kingdom2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kunz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cao","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Aviron, Mountain View, California 940343; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoinette","family":"Tishon","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Lewicki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Campbell","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Howard Hughes Medical Institute, Departments of Physiology, Biophysics, and Neurology, University of Iowa College of Medicine, Iowa City, Iowa 522424"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael B. A.","family":"Oldstone","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.521"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.657"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.12.7270-7281.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.2.1059-1070.1995"},{"key":"e_1_3_2_6_2","unstructured":"Borrow P. Oldstone M. B. A. Lymphocytic choriomeningitis virus Viral pathogenesis. 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