{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T13:01:25Z","timestamp":1769346085835,"version":"3.49.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,2,15]],"date-time":"2001-02-15T00:00:00Z","timestamp":982195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia virus types 1 and 2, can induce persistent nonneoplastic expansion of the CD5<jats:sup>+<\/jats:sup>B-cell population, termed persistent lymphocytosis (PL). As in human CD5<jats:sup>+<\/jats:sup>B cells, we report here that CD5 was physically associated with the B-cell receptor (BCR) in normal bovine CD5<jats:sup>+<\/jats:sup>B cells. In contrast, in CD5<jats:sup>+<\/jats:sup>B cells from BLV-infected PL cattle, CD5 was dissociated from the BCR. In B cells from PL cattle, apoptosis decreased when cells were stimulated with antibody to surface immunoglobulin M (sIgM), while in B cells from uninfected cattle, apoptosis increased after sIgM stimulation. The functional significance of the CD5-BCR association was suggested by experimental dissociation of the CD5-BCR interaction by cross-linking of CD5. This caused CD5<jats:sup>+<\/jats:sup>B cells from uninfected animals to decrease apoptosis when stimulated with anti-sIgM. In contrast, in CD5<jats:sup>+<\/jats:sup>B cells from PL animals, in which CD5 was already dissociated from the BCR, there was no statistically significant change in apoptosis when CD5 was cross-linked and the cells were stimulated with anti-sIgM. Disruption of CD5-BCR interactions and subsequent decreased apoptosis and increased survival in antigenically stimulated B cells may be a mechanism of BLV-induced PL.<\/jats:p>","DOI":"10.1128\/jvi.75.4.1689-1696.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"1689-1696","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["CD5 Is Dissociated from the B-Cell Receptor in B Cells from Bovine Leukemia Virus-Infected, Persistently Lymphocytotic Cattle: Consequences to B-Cell Receptor-Mediated Apoptosis"],"prefix":"10.1128","volume":"75","author":[{"given":"Glenn H.","family":"Cantor","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne M.","family":"Pritchard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck","family":"Dequiedt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Applied Biochemistry and Biology, Molecular Biology and Animal Physiology Unit, Faculty of Agronomy, B-5030 Gembloux, Belgium2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Willems","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Applied Biochemistry and Biology, Molecular Biology and Animal Physiology Unit, Faculty of Agronomy, B-5030 Gembloux, Belgium2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Kettmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Applied Biochemistry and Biology, Molecular Biology and Animal Physiology Unit, Faculty of Agronomy, B-5030 Gembloux, Belgium2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Davis","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.9.5845-5853.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.1992.tb01459.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5294.1906"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9035"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9311"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1999.1475"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230922"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7716523"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199805)28:05<1617::AID-IMMU1617>3.0.CO;2-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(89)90071-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1127-1137.1999"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.1.630-639.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618087"},{"key":"e_1_3_2_15_2","first-page":"705","article-title":"Relationship between lymphosarcoma and persistent lymphocytosis in cattle: a review","volume":"175","author":"Ferrer J. 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