{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:26:18Z","timestamp":1761647178729},"reference-count":45,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2001,3,15]],"date-time":"2001-03-15T00:00:00Z","timestamp":984614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Thymomas induced by polyomavirus strain PTA in mice are known to express the major capsid protein VP-1. Since the expression of a late structural protein such as VP-1 is considered a sign of virus replication, the present work attempted to clarify the implication of the presence of this protein in tumor cells. Electron microscopy of tumors showed a striking absence of viral particles in the vast majority of the cells. However, immunoelectron microscopy of the same samples demonstrated intranuclear VP-1 in most cells despite the absence of viral particles. Very little infectious virus was recovered from tumors. A change in the electrophoretic mobility of VP-1 from thymomas was detected compared with VP-1 from productively infected cells. The data presented in this work prove that the expression of VP-1 in polyomavirus-induced tumors is not synonymous with the presence of infectious virus, suggesting a possible defect in viral encapsidation.<\/jats:p>","DOI":"10.1128\/jvi.75.6.2891-2899.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"2891-2899","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Expression of Major Capsid Protein VP-1 in the Absence of Viral Particles in Thymomas Induced by Murine Polyomavirus"],"prefix":"10.1128","volume":"75","author":[{"given":"Norberto","family":"Sanjuan","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Experimental Pathology, Department of Microbiology, University of Buenos Aires School of Medicine, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anal\u0131\u0301a","family":"Porra\u0301s","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Experimental Pathology, Department of Microbiology, University of Buenos Aires School of Medicine, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Otero","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Experimental Pathology, Department of Microbiology, University of Buenos Aires School of Medicine, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sof\u0131\u0301a","family":"Perazzo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratory of Experimental Pathology, Department of Microbiology, University of Buenos Aires School of Medicine, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.7.5826-5832.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1213-1218.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8596"},{"key":"e_1_3_2_5_2","unstructured":"Cole\nC. 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