{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:08:34Z","timestamp":1789751314768,"version":"4.0.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by a reactivation of the polyomavirus JC (JCV) within a setting of immunosuppression. The nature of the immune response that contains replication of this virus is unknown. We have explored JCV-specific cellular immune responses in patients with PML and control subjects. JCV antigen-stimulated peripheral blood mononuclear cells (PBMC) of four human immunodeficiency virus (HIV)-infected patients who were survivors of PML and one HIV-uninfected patient recently diagnosed with PML lysed autologous B-lymphoblastoid cell lines expressing either the JCV T regulatory protein or the VP1 major capsid protein. This lysis was mediated by CD8\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes and was major histocompatibility complex class I restricted. These cells were therefore cytotoxic T lymphocytes (CTL). JCV-specific CTL could not be detected in PBMC of three HIV-infected PML patients who had progressive neurologic disease and an eventual fatal outcome. These data suggest that the JCV-specific cellular immune response may play a crucial role in the containment of PML. This finding may also prove useful as a favorable prognostic marker in the clinical management of these patients.\n                  <\/jats:p>","DOI":"10.1128\/jvi.75.7.3483-3487.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:58:35Z","timestamp":1027749515000},"page":"3483-3487","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["JC Virus-Specific Cytotoxic T Lymphocytes in Individuals with Progressive Multifocal Leukoencephalopathy"],"prefix":"10.1128","volume":"75","author":[{"given":"Igor J.","family":"Koralnik","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Neurology1 and"},{"name":"<!--label omitted: 2-->Division of Viral Pathogenesis, Department of Medicine,2 Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud A.","family":"Du Pasquier","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Neurology1 and"},{"name":"<!--label omitted: 2-->Division of Viral Pathogenesis, Department of Medicine,2 Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman L.","family":"Letvin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Viral Pathogenesis, Department of Medicine,2 Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199205000-00003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.8.1194"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.2.462"},{"key":"e_1_3_2_5_2","first-page":"289","article-title":"Human polyomavirus in pregnancy. A model for the study of defence mechanisms to virus reactivation","volume":"53","author":"Coleman D. V.","year":"1983","unstructured":"Coleman D. V. Gardner S. D. Mulholland C. Fridiksdottir V. Porter A. A. Lilford R. Valdimarsson H. Human polyomavirus in pregnancy. A model for the study of defence mechanisms to virus reactivation.Clin. Exp. Immunol. 53 1983 289 296","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.33.1.319-321.1981"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196907000-00009"},{"key":"e_1_3_2_8_2","first-page":"451","article-title":"Humoral and cellular immune response to influenza virus vaccination in aged humans","volume":"5","author":"Fagiolo U.","year":"1993","unstructured":"Fagiolo U. Amadori A. Cozzi E. Bendo R. Lama M. Douglas A. Palu G. 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