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FIV RNA was detected in tonsil and oral or vaginal mucosa as early as 1 day p.i. by TSA-ISH and in retropharyngeal, tracheobronchial, or external iliac lymph nodes and sometimes in spleen or blood mononuclear cells by day 2, indicating that regional and distant spread of virus-infected cells occurred rapidly after mucosal exposure. By day 8, viral RNA, DNA, and culturable virus were uniformly detected in regional and distant tissues, connoting systemic infection. TSA-ISH proved more sensitive than DNA PCR in detecting early FIV-infected cells. In mucosal tissues, the earliest demonstrable FIV-bearing cells were either within or subjacent to the mucosal epithelium or were in germinal centers of regional lymph nodes. The FIV<jats:sup>+<\/jats:sup>cells were of either of two morphological types, large stellate or small round. Those FIV RNA<jats:sup>+<\/jats:sup>cells which could be colabeled for a phenotype marker, were labeled for either dendritic-cell-associated protein p55 or T-lymphocyte receptor antigen CD3. These studies indicate that FIV crosses mucous membranes within hours after exposure and rapidly traffics via dendritic and T cells to systemic lymphoid tissues, a pathway similar to that thought to occur in the initial phase of infection by the human and simian immunodeficiency viruses.<\/jats:p>","DOI":"10.1128\/jvi.76.12.6311-6322.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"6311-6322","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Early Pathogenesis of Transmucosal Feline Immunodeficiency Virus Infection"],"prefix":"10.1128","volume":"76","author":[{"given":"Leslie A.","family":"Obert","sequence":"first","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward A.","family":"Hoover","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.1.209-213.1987"},{"key":"e_1_3_2_3_2","first-page":"760","volume":"4","year":"1991","unstructured":"Amerongen, H. 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