{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:24:11Z","timestamp":1791199451574,"version":"4.2.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The 3\u2032-terminal sequences of hepatitis C virus (HCV) positive- and negative-strand RNAs contribute\n            <jats:italic>cis<\/jats:italic>\n            -acting functions essential for viral replication. The secondary structure and protein-binding properties of these highly conserved regions are of interest not only for the further elucidation of HCV molecular biology, but also for the design of antisense therapeutic constructs. The RNA structure of the positive-strand 3\u2032 untranslated region has been shown previously to influence binding by various host and viral proteins and is thus thought to promote HCV RNA synthesis and genome stability. Recent studies have attributed analogous functions to the negative-strand 3\u2032 terminus. We evaluated the HCV negative-strand secondary structure by enzymatic probing with single-strand-specific RNases and thermodynamic modeling of RNA folding. The accessibility of both 3\u2032-terminal sequences to hybridization by antisense constructs was evaluated by RNase H cleavage mapping in the presence of combinatorial oligodeoxynucleotide libraries. The mapping results facilitated identification of antisense oligodeoxynucleotides and a 10-23 deoxyribozyme active against the positive-strand 3\u2032-X region RNA in vitro.\n          <\/jats:p>","DOI":"10.1128\/jvi.76.19.9563-9574.2002","type":"journal-article","created":{"date-parts":[[2002,9,3]],"date-time":"2002-09-03T23:18:19Z","timestamp":1031095099000},"page":"9563-9574","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Secondary Structure and Hybridization Accessibility of Hepatitis C Virus 3\u2032-Terminal Sequences"],"prefix":"10.1128","volume":"76","author":[{"given":"Robert M.","family":"Smith","sequence":"first","affiliation":[{"name":"Division of Gastroenterology-Hepatology, Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cherie M.","family":"Walton","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology-Hepatology, Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine H.","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology-Hepatology, Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George Y.","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology-Hepatology, Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.21.4113"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280236"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.4.1708-1721.2001"},{"key":"e_1_3_2_5_2","first-page":"429","volume":"3","year":"1997","unstructured":"Birikh, K. 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