{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:58:22Z","timestamp":1790974702525,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2002,4,15]],"date-time":"2002-04-15T00:00:00Z","timestamp":1018828800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2002,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Endoplasmic reticulum (ER) \u03b1-glucosidase inhibitors, which block the trimming step of N-linked glycosylation, have been shown to eliminate the production of several ER-budding viruses. Here we investigated the effects of one such inhibitor,\n            <jats:italic>N<\/jats:italic>\n            -nonyl-deoxynojirimycin (\n            <jats:italic>N<\/jats:italic>\n            N-DNJ), a 9-carbon alkyl iminosugar derivative, on infection by Japanese encephalitis virus (JEV) and dengue virus serotype 2 (DEN-2). In the presence of\n            <jats:italic>N<\/jats:italic>\n            N-DNJ, JEV and DEN-2 infections were suppressed in a dose-dependent manner. This inhibitory effect appeared to influence DEN-2 infection more than JEV infection, since lower concentrations of\n            <jats:italic>N<\/jats:italic>\n            N-DNJ substantially blocked DEN-2 replication. Secretion of the flaviviral glycoproteins E and NS1 was greatly reduced, and levels of DEN-2 viral RNA replication measured by fluorogenic reverse transcription-PCR were also decreased, by\n            <jats:italic>N<\/jats:italic>\n            N-DNJ. Notably, the viral glycoproteins, prM, E, and NS1 were found to associate transiently with the ER chaperone calnexin, and this interaction was affected by\n            <jats:italic>N<\/jats:italic>\n            N-DNJ, suggesting a potential role of calnexin in the folding of flaviviral glycoproteins. Additionally, in a mouse model of lethal challenge by JEV infection, oral delivery of\n            <jats:italic>N<\/jats:italic>\n            N-DNJ reduced the mortality rate. These findings show that\n            <jats:italic>N<\/jats:italic>\n            N-DNJ has an antiviral effect on flavivirus infection, likely through interference with virus replication at the posttranslational modification level, occurring mainly in the ER.\n          <\/jats:p>","DOI":"10.1128\/jvi.76.8.3596-3604.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"3596-3604","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":199,"title":["Antiviral Effects of an Iminosugar Derivative on Flavivirus Infections"],"prefix":"10.1128","volume":"76","author":[{"given":"Shu-Fen","family":"Wu","sequence":"first","affiliation":[{"name":"Graduate Institute of Life Sciences"},{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chyan-Jang","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences"},{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Len","family":"Liao","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences"},{"name":"Department of Microbiology and Immunology, National Defense Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond A.","family":"Dwek","sequence":"additional","affiliation":[{"name":"Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Zitzmann","sequence":"additional","affiliation":[{"name":"Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ling","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences"},{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of 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