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We considered the<jats:italic>Runx3<\/jats:italic>gene a potential candidate on the basis of genetic mapping which showed that<jats:italic>Dsi1<\/jats:italic>and<jats:italic>Runx3<\/jats:italic>are closely linked on mouse chromosome 4 and the precedent of the related<jats:italic>Runx2<\/jats:italic>gene, which emerged recently as a<jats:italic>Myc<\/jats:italic>-collaborating gene activated by retroviral insertion in thymic lymphomas of CD2-<jats:italic>MYC<\/jats:italic>mice. We now report the physical mapping of the<jats:italic>Dsi1<\/jats:italic>locus to a site 30 kb upstream of the distal (P1) promoter of the murine<jats:italic>Runx3<\/jats:italic>gene. Comparison with the syntenic region of human chromosome 1 shows that the next gene is over 250 kb 5\u2032 to<jats:italic>Runx3<\/jats:italic>, suggesting that<jats:italic>Runx3<\/jats:italic>may be the primary target of retroviral insertions at<jats:italic>Dsi1<\/jats:italic>. Screening of CD2-<jats:italic>MYC<\/jats:italic>lymphomas for rearrangements at<jats:italic>Dsi1<\/jats:italic>revealed a tumor cell line harboring an MLV provirus at this locus, in the orientation opposite that of<jats:italic>Runx3<\/jats:italic>. Proviral insertion was associated with very high levels of expression of<jats:italic>Runx3<\/jats:italic>, with a preponderance of transcripts arising at the P1 promoter. These results confirm that<jats:italic>Runx3<\/jats:italic>is a target of retroviral insertions at<jats:italic>Dsi1<\/jats:italic>and indicate that<jats:italic>Runx3<\/jats:italic>can act as an alternative to<jats:italic>Runx2<\/jats:italic>as a<jats:italic>Myc<\/jats:italic>-collaborating gene in thymic lymphoma.<\/jats:p>","DOI":"10.1128\/jvi.76.9.4364-4369.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"4364-4369","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["The Common Retroviral Insertion Locus<i>Dsi1<\/i>Maps 30 Kilobases Upstream of the P1 Promoter of the Murine<i>Runx3<\/i>\/<i>Cbfa3<\/i>\/<i>Aml2<\/i>Gene"],"prefix":"10.1128","volume":"76","author":[{"given":"Monica","family":"Stewart","sequence":"first","affiliation":[{"name":"Molecular Oncology Laboratory, Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow G61 1QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"MacKay","sequence":"additional","affiliation":[{"name":"Molecular Oncology Laboratory, Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow G61 1QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewan R.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Molecular Oncology Laboratory, Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow G61 1QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Neil","sequence":"additional","affiliation":[{"name":"Molecular Oncology Laboratory, Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow G61 1QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00751-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00060-J"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.9.8367"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.11.4.1820-1828.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.20.9790-9798.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(87)90294-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204090"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(95)80184-N"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(89)90111-0"},{"key":"e_1_3_2_11_2","first-page":"1009","volume":"8","year":"1989","unstructured":"Dolcetti, R., S. 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