{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:19:11Z","timestamp":1790857151015,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2003,8,15]],"date-time":"2003-08-15T00:00:00Z","timestamp":1060905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2003,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    RNA replicons derived from flavivirus genomes show considerable potential as gene transfer and immunization vectors. A convenient and efficient encapsidation system is an important prerequisite for the practical application of such vectors. In this work, tick-borne encephalitis (TBE) virus replicons and an appropriate packaging cell line were constructed and characterized. A stable CHO cell line constitutively expressing the two surface proteins prM\/M and E (named CHO-ME cells) was generated and shown to efficiently export mature recombinant subviral particles (RSPs). When replicon Nd\u0394ME lacking the prM\/M and E genes was introduced into CHO-ME cells, virus-like particles (VLPs) capable of initiating a single round of infection were released, yielding titers of up to 5 \u00d7 10\n                    <jats:sup>7<\/jats:sup>\n                    \/ml in the supernatant of these cells. Another replicon (Nd\u0394CME) lacking the region encoding most of the capsid protein C in addition to proteins prM\/M and E was not packaged by CHO-ME cells. As observed with other flavivirus replicons, both TBE virus replicons appeared to exert no cytopathic effect on their host cells. Sedimentation analysis revealed that the Nd\u0394ME-containing VLPs were physically distinct from RSPs and similar to infectious virions. VLPs could be repeatedly passaged in CHO-ME cells but maintained the property of being able to initiate only a single round of infection in other cells during these passages. CHO-ME cells can thus be used both as a source for mature TBE virus RSPs and as a safe and convenient replicon packaging cell line, providing the TBE virus surface proteins prM\/M and E in\n                    <jats:italic>trans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/jvi.77.16.8924-8933.2003","type":"journal-article","created":{"date-parts":[[2003,7,28]],"date-time":"2003-07-28T17:36:47Z","timestamp":1059413807000},"page":"8924-8933","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Incorporation of Tick-Borne Encephalitis Virus Replicons into Virus-Like Particles by a Packaging Cell Line"],"prefix":"10.1128","volume":"77","author":[{"given":"Rainer","family":"Gehrke","sequence":"first","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Ecker","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan W.","family":"Aberle","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Allison","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz X.","family":"Heinz","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian W.","family":"Mandl","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6756","DOI":"10.4049\/jimmunol.163.12.6756","volume":"163","year":"1999","unstructured":"Aberle, J. 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