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The<jats:italic>M2<\/jats:italic>mutant showed no defect in productive replication in vitro or in lungs after infection of mice. Likewise, the characteristic transient increase in spleen cell number, V\u03b24 T-cell-receptor-positive CD8<jats:sup>+<\/jats:sup>T-cell mononucleosis, and establishment of latency were unaffected. However, the<jats:italic>M2<\/jats:italic>mutant virus was defective in its ability to cause the transient sharp rise in latently infected cells normally seen in the spleen after infection of mice. We also demonstrate that expression of<jats:italic>M2<\/jats:italic>is restricted to B cells in the spleen and that<jats:italic>M2<\/jats:italic>encodes a 30-kDa protein localizing predominantly in the cytoplasm and plasma membrane of B cells.<\/jats:p>","DOI":"10.1128\/jvi.77.17.9700-9709.2003","type":"journal-article","created":{"date-parts":[[2003,8,14]],"date-time":"2003-08-14T22:53:50Z","timestamp":1060901630000},"page":"9700-9709","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Murid Herpesvirus 4 Strain 68 M2 Protein Is a B-Cell-Associated Antigen Important for Latency but Not Lymphocytosis"],"prefix":"10.1128","volume":"77","author":[{"given":"Alastair I.","family":"Macrae","sequence":"first","affiliation":[{"name":"Laboratory for Clinical and Molecular Virology, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Usherwood","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth College Medical School, Hanover, New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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