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We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By systematic mutational analysis of the LCMV GP cleavage site, we determined that the consensus motif R-(R\/K\/H)-L-(A\/L\/S\/T\/F)\n            <jats:sub>265<\/jats:sub>\n            is essential for the endoproteolytic processing. In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1\/S1P cleaves LCMV GP.\n          <\/jats:p>","DOI":"10.1128\/jvi.77.5.2866-2872.2003","type":"journal-article","created":{"date-parts":[[2003,2,12]],"date-time":"2003-02-12T20:46:14Z","timestamp":1045082774000},"page":"2866-2872","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":202,"title":["Endoproteolytic Processing of the Lymphocytic Choriomeningitis Virus Glycoprotein by the Subtilase SKI-1\/S1P"],"prefix":"10.1128","volume":"77","author":[{"given":"Winfried R.","family":"Beyer","sequence":"first","affiliation":[{"name":"Heinrich-Pette-Institut fu\u0308r Experimentelle Virologie und Immunologie an der Universita\u0308t Hamburg, D-20251 Hamburg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Po\u0308pplau","sequence":"additional","affiliation":[{"name":"Heinrich-Pette-Institut fu\u0308r Experimentelle Virologie und Immunologie an der Universita\u0308t Hamburg, D-20251 Hamburg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Garten","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Virologie, Philipps Universita\u0308t, 35037 Marburg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee","family":"von Laer","sequence":"additional","affiliation":[{"name":"Georg-Speyer-Haus, 60596 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Lenz","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Virologie, Philipps Universita\u0308t, 35037 Marburg"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.0909"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.52.3.897-904.1984"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90438-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02394-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.22.11905"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.2.1061-1064.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.3.1488-1495.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.12.7270-7281.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11041"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90135-6"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(79)90042-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"Burns J. 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