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We report here the identification of a 14-amino-acid peptide, SAGKVSSTLASELG, derived from the MV nucleoprotein (MV-N) bound to HLA-DRB1*0301. Peripheral blood mononuclear cells (PBMC) from 281 previously vaccinated measles-mumps-rubella II (MMR-II) subjects (HLA discordant) were studied for peptide recognition by T cells. Significant gamma interferon (IFN-\u03b3) responses to MV-P and MV-N peptides were observed in 55.9 and 15.3% of subjects, respectively. MV-P- and MV-N-specific interleukin-4 (IL-4) responses were detected in 19.2 and 23.1%, respectively, of PBMC samples. Peptide-specific cytokine responses and HLA-DRB1 allele associations revealed that, for the MV-P peptide, the allele with the strongest association with both IFN-\u03b3 (<jats:italic>P<\/jats:italic>= 0.02) and IL-4 (<jats:italic>P<\/jats:italic>= 0.03) secretion was DRB1*0301. For MV-N, the allele with the strongest association with IFN-\u03b3 secretion was DRB1*1501 (<jats:italic>P<\/jats:italic>= 0.04), and the alleles with the strongest associations with IL-4 secretion were DRB1*1103 and DRB1*1303 (<jats:italic>P<\/jats:italic>= 0.01). These results indicate that HLA class II MV proteins can be processed, presented, and identified, and the ability to generate cell-mediated immune responses can be demonstrated. This information is promising for new vaccine design strategies with peptide-based vaccines.<\/jats:p>","DOI":"10.1128\/jvi.78.1.42-51.2004","type":"journal-article","created":{"date-parts":[[2003,12,11]],"date-time":"2003-12-11T23:43:13Z","timestamp":1071186193000},"page":"42-51","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Identification and Characterization of Novel, Naturally Processed Measles Virus Class II HLA-DRB1 Peptides"],"prefix":"10.1128","volume":"78","author":[{"given":"Inna G.","family":"Ovsyannikova","sequence":"first","affiliation":[{"name":"Mayo Vaccine Research Group"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"The W. M. 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