{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:37:59Z","timestamp":1791153479800,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2004,7,15]],"date-time":"2004-07-15T00:00:00Z","timestamp":1089849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2004,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Adenovirus serotype 37 (Ad37) belongs to species D and can cause epidemic keratoconjunctivitis, whereas the closely related Ad19p does not. Primary cell attachment by adenoviruses is mediated through receptor binding of the knob domain of the fiber protein. The knobs of Ad37 and Ad19p differ at only two positions, Lys240Glu and Asn340Asp. We report the high-resolution crystal structures of the Ad37 and Ad19p knobs, both native and in complex with sialic acid, which has been proposed as a receptor for Ad37. Overall, the Ad37 and Ad19p knobs are very similar to previously reported knob structures, especially to that of Ad5, which binds the coxsackievirus-adenovirus receptor (CAR). Ad37 and Ad19p knobs are structurally identical with the exception of the changed side chains and are structurally most similar to CAR-binding knobs (e.g., that of Ad5) rather than non-CAR-binding knobs (e.g., that of Ad3). The two mutations in Ad19p result in a partial loss of the exceptionally high positive surface charge of the Ad37 knob but do not affect sialic acid binding. This site is located on the top of the trimer and binds both \u03b1(2,3) and \u03b1(2,6)-linked sialyl-lactose, although only the sialic acid residue makes direct contact. Amino acid alignment suggests that the sialic acid binding site is conserved in several species D serotypes. Our results show that the altered viral tropism and cell binding of Ad19p relative to those of Ad37 are not explained by a different binding ability toward sialyl-lactose.<\/jats:p>","DOI":"10.1128\/jvi.78.14.7727-7736.2004","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T19:18:20Z","timestamp":1088191100000},"page":"7727-7736","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":158,"title":["Crystal Structure of Species D Adenovirus Fiber Knobs and Their Sialic Acid Binding Sites"],"prefix":"10.1128","volume":"78","author":[{"given":"Wim P.","family":"Burmeister","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie Mole\u0301culaire et Structurale, EA 2939, Universite\u0301 Joseph Fourier, Faculte\u0301 de Me\u0301decine de Grenoble, F-38706 La Tronche"},{"name":"EMBL, Grenoble Outstation, F-38042 Grenoble cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Guilligay","sequence":"additional","affiliation":[{"name":"EMBL, Grenoble Outstation, F-38042 Grenoble cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Cusack","sequence":"additional","affiliation":[{"name":"EMBL, Grenoble Outstation, F-38042 Grenoble cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Go\u0308ran","family":"Wadell","sequence":"additional","affiliation":[{"name":"Department of Virology, Umea\u030a University, SE-90185 Umea\u030a, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Arnberg","sequence":"additional","affiliation":[{"name":"Department of Virology, Umea\u030a University, SE-90185 Umea\u030a, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.13.4023"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1503"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.17.8834-8841.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.16.7691-7693.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.1.42-48.2000"},{"key":"e_1_3_2_7_2","first-page":"239","volume":"6","year":"1997","unstructured":"Arnberg, N., Y. 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