{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T21:45:41Z","timestamp":1763070341468},"reference-count":60,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>An ideal vaccine delivery system would elicit persistent protection following a single administration, preferably by a noninvasive route, and be safe even in the face of immunosuppression, either inherited or acquired, of the recipient. We have exploited the unique life cycle of the autonomous parvoviruses to develop a nonproliferating vaccine platform that appears to both induce priming and continually boost a protective immune response following a single inoculation. A crippled parvovirus vector was constructed, based on a chimera between minute virus of mice (MVM) and LuIII, which expresses<jats:italic>Borrelia burgdorferi<\/jats:italic>outer surface protein A (OspA) instead of its coat protein. The vector was packaged into an MVM lymphotropic capsid and inoculated into naive C3H\/HeNcr mice. Vaccination with a single vector dose, either intravenously or intranasally, elicited high-titer anti-OspA-specific antibody that provided protection from live spirochete challenge and was sustained over the lifetime of the animal. Both humoral and cell-mediated Th<jats:sub>1<\/jats:sub>immunity was induced, as shown by anti-OspA immunoglobulin G2a antibody and preferential gamma interferon production by OspA-specific CD4<jats:sup>+<\/jats:sup>T cells.<\/jats:p>","DOI":"10.1128\/jvi.78.3.1101-1108.2004","type":"journal-article","created":{"date-parts":[[2004,1,13]],"date-time":"2004-01-13T21:28:00Z","timestamp":1074029280000},"page":"1101-1108","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["A Nonproliferating Parvovirus Vaccine Vector Elicits Sustained, Protective Humoral Immunity following a Single Intravenous or Intranasal Inoculation"],"prefix":"10.1128","volume":"78","author":[{"given":"Gene A.","family":"Palmer","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Brogdon","sequence":"additional","affiliation":[{"name":"Immunobiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie L.","family":"Constant","sequence":"additional","affiliation":[{"name":"Immunobiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Tattersall","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"},{"name":"Genetics, Yale University School of Medicine, New Haven, Connecticut 06510"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm732"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00076-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.249"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6718-6724.2001"},{"key":"e_1_3_2_6_2","first-page":"521","volume":"57","year":"1984","unstructured":"Barbour, A. G. 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med.57:521-525.","journal-title":"Yale J. Biol. Med."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.133"},{"key":"e_1_3_2_8_2","first-page":"959","volume":"143","year":"1993","unstructured":"Barthold, S. W., M. S. de Souza, J. L. Janotka, A. L. Smith, and D. H. Persing. 1993. Chronic Lyme borreliosis in the laboratory mouse. Am. J. Pathol.143:959-971.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_2_9_2","first-page":"263","volume":"139","year":"1991","unstructured":"Barthold, S. W., D. H. Persing, A. L. Armstrong, and R. A. Peeples. 1991. Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice. Am. J. Pathol.139:263-273.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6445-6455.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-145-1-211"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4724"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303402320987833"},{"key":"e_1_3_2_14_2","first-page":"357","volume":"65","year":"1991","unstructured":"Brownstein, D. G., A. L. Smith, R. O. Jacoby, E. A. Johnson, G. Hansen, and P. Tattersall. 1991. Pathogenesis of infection with a virulent allotropic variant of minute virus of mice and regulation by host genotype. Lab. Investig.65:357-364.","journal-title":"Lab. Investig."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1991.tb17255.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301976"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.10.3311"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700496"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00739.x"},{"key":"e_1_3_2_20_2","first-page":"658","volume":"3","year":"1996","unstructured":"Conrad, C. K., S. S. Allen, S. A. Afione, T. C. Reynolds, S. E. Beck, M. Fee-Maki, X. Barrazza-Ortiz, R. Adams, F. B. Askin, B. J. Carter, W. B. Guggino, and T. R. Flotte. 1996. Safety of single-dose administration of an adeno-associated virus (adeno-associated virus)-CFTR vector in the primate lung. Gene Ther.3:658-668.","journal-title":"Gene Ther."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.297"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9520"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301477"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1397-1406.1994"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1453"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2000.0032"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.6.2553-2557.1993"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.8.2605-2613.1988"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.12.3733"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8549-8558.1999"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/00125817-200211001-00012"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.59.1.8-13.1986"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.5.1374"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"6742","DOI":"10.4049\/jimmunol.151.12.6742","volume":"151","year":"1993","unstructured":"Levin, D., S. Constant, T. Pasqualini, R. Flavell, and K. Bottomly. 1993. Role of dendritic cells in the priming of CD4+ T lymphocytes to peptide antigen in vivo. J. Immunol.151:6742-6750.","journal-title":"J. Immunol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.6.2888-2894.2000"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700391"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.681-686.2001"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(00)01810-4"},{"key":"e_1_3_2_39_2","first-page":"374","volume":"3","year":"2002","unstructured":"Newman, M. J. 2002. Heterologous prime-boost vaccination strategies for HIV-1: augmenting cellular immune responses. Curr. Opin. Investig. Drugs3:374-378.","journal-title":"Curr. Opin. Investig. Drugs"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566523023347797"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","unstructured":"Palmer G. A. and P. Tattersall. 2000. Autonomous parvoviruses as gene transfer vectors p. 178-200. In S. Faisst and J. Rommelaere (ed.) Contributions to microbiology vol. 4: parvoviruses. Karger Press Basel Switzerland.","DOI":"10.1159\/000060337"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.21594"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.25.3.1003-1007.1979"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.19.9493-9501.2001"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(00)01612-1"},{"key":"e_1_3_2_46_2","unstructured":"Rommelaere J. and P. Tattersall. 1990. Oncosuppression by parvoviruses p. 41-58. In P. Tjissen (ed.) Handbook of parvoviruses vol. II. CRC Press Boca Raton Fla."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.5.2821-2828.1992"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.4049\/jimmunol.139.8.2708","volume":"139","year":"1987","unstructured":"Samelson, L. E., J. J. O'Shea, H. Luong, P. Ross, K. B. Urdahl, R. D. Klausner, and J. Bluestone. 1987. T-cell antigen receptor phosphorylation induced by an anti-receptor antibody. J. Immunol.139:2708-2714.","journal-title":"J. Immunol."},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.1.269-277.2001"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.233"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.5.3229-3232.1995"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3107127"},{"key":"e_1_3_2_53_2","first-page":"152","volume":"50","year":"2003","unstructured":"Stilwell, J. L., and R. J. Samulski. 2003. Adeno-associated virus vectors for therapeutic gene transfer. BioTechniques50:152-154.","journal-title":"BioTechniques"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.10.4.586-590.1972"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.46.3.944-955.1983"},{"key":"e_1_3_2_56_2","unstructured":"Tattersall P. and E. M. Gardiner. 1990. Autonomous parvovirus-host interactions p. 111-122. In P. Tjissen (ed.) Handbook of parvoviruses vol. I. CRC Press Boca Raton Fla."},{"key":"e_1_3_2_57_2","first-page":"174","volume":"6","year":"2003","unstructured":"Wright, J. F., G. Qu, C. Tang, and J. M. Sommer. 2003. Recombinant adeno-associated virus: formulation challenges and strategies for a gene therapy vector. Curr. Opin. Drug Discov. Dev.6:174-178.","journal-title":"Curr. Opin. Drug Discov. Dev."},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.6.3851-3858.2003"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2000.0045"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430340260201662"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.9.4580-4590.2002"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.3.1101-1108.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.3.1101-1108.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T18:33:11Z","timestamp":1682620391000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.78.3.1101-1108.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":60,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1128\/JVI.78.3.1101-1108.2004"],"URL":"https:\/\/doi.org\/10.1128\/jvi.78.3.1101-1108.2004","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2]]},"assertion":[{"value":"2003-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}