{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:59:39Z","timestamp":1790967579748,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,2,15]],"date-time":"2004-02-15T00:00:00Z","timestamp":1076803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2004,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            LMP1 is an Epstein-Barr virus (EBV)-encoded membrane protein essential for the proliferation of EBV-infected lymphoblasts (E. Kilger, A. Kieser, M. Baumann, and W. Hammerschmidt, EMBO J.\n            <jats:bold>17:<\/jats:bold>\n            1700-1709, 1998). LMP1 also inhibits gene expression and induces cytostasis in transfected cells when it is expressed at levels as little as twofold higher than the average for EBV-positive lymphoblasts (M. Sandberg, A. Kaykas, and B. Sugden, J. Virol.\n            <jats:bold>74:<\/jats:bold>\n            9755-9761, 2000; A. Kaykas and B. Sugden, Oncogene\n            <jats:bold>19:<\/jats:bold>\n            1400-1410, 2000). We have found that in three different clones of EBV-infected lymphoblasts the levels of expression of LMP1 in individual cells in each clone ranged over 100-fold. This difference is due to a difference in levels of the LMP1 transcript. In these clones, cells expressing high levels of LMP1 incorporated less BrdU. We also found that induction of expression of LMP1 or of a derivative of LMP1 with its transmembrane domain fused to green fluorescent protein instead of its carboxy-terminal signaling domain resulted in phosphorylation of eIF2\u03b1 in EBV-negative Burkitt's lymphoma cells. This induction of phosphorylation of eIF2\u03b1 was also detected in EBV-infected lymphoblasts, in which high levels of LMP1 correlated with high levels of phosphorylation of eIF2\u03b1. Our results indicate that inhibition of gene expression and of cell proliferation by LMP1 occurs normally in EBV-infected cells.\n          <\/jats:p>","DOI":"10.1128\/jvi.78.4.1657-1664.2004","type":"journal-article","created":{"date-parts":[[2004,1,27]],"date-time":"2004-01-27T19:58:01Z","timestamp":1075233481000},"page":"1657-1664","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["High Physiological Levels of LMP1 Result in Phosphorylation of eIF2\u03b1 in Epstein-Barr Virus-Infected Cells"],"prefix":"10.1128","volume":"78","author":[{"given":"Ngan","family":"Lam","sequence":"first","affiliation":[{"name":"McArdle Laboratory for Cancer Research, University of Wisconsin\u2014Madison, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark L.","family":"Sandberg","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, University of Wisconsin\u2014Madison, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bill","family":"Sugden","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, University of Wisconsin\u2014Madison, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"67","volume":"4","year":"1989","unstructured":"Baichwal, V. R., and B. Sugden. 1989. The multiple membrane-spanning segments of the BNLF-1 oncogene from Epstein-Barr virus are required for transformation. Oncogene4:67-74.","journal-title":"Oncogene"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00780.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005461200"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)88975-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.6.3749-3758.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.5.1833-1840.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.10.12.8903508"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.4.1907"},{"key":"e_1_3_2_10_2","first-page":"2982","volume":"63","year":"2003","unstructured":"Dirmeier, U., B. Neuhierl, E. Kilger, G. Reisbach, M. L. Sandberg, and W. Hammerschmidt. 2003. Latent membrane protein 1 is critical for efficient growth transformation of human B cells by Epstein-Barr virus. 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