{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:39:38Z","timestamp":1790915978056,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2004,4,15]],"date-time":"2004-04-15T00:00:00Z","timestamp":1081987200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2004,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Subgroup D adenovirus (Ad) types 8, 19, and 37 (Ad8, -19, and -37, respectively) are causative agents of epidemic keratoconjunctivitis and genital tract infections. Previous studies showed that Ad37 binds to a 50-kDa membrane glycoprotein expressed on human ocular (conjunctival) cells. To identify and characterize the role of the 50-kDa glycoprotein in Ad37 infection, we partially purified this molecule from solubilized Chang C conjunctival cell membranes by using lentil lectin chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Liquid chromatography coupled to nano-electrospray ionization-tandem mass spectrometry was subsequently used to identify four Ad37 receptor candidates: CD46, CD87, CD98, and CD147. Immunodepletion analyses demonstrated that the 50-kDa protein is identical to CD46 (also known as membrane cofactor protein). The Ad37, but not Ad5, fiber knob bound to the extracellular domain of CD46, demonstrating a direct interaction of an Ad37 capsid protein with CD46. An antibody specific for the N-terminal 19 amino acids of CD46 also blocked Ad37 infection of human cervical carcinoma and conjunctival cells, indicating a requirement for CD46 in infection. Finally, expression of a 50-kDa isoform of human CD46 in a CD46-null cell line increased cell binding by wild-type Ad37 and gene delivery by an Ad vector pseudotyped with the Ad37 fiber, but not by a vector bearing the Ad5 fiber. Together, these studies demonstrate that CD46 serves as an attachment receptor for Ad37 and shed further light on the cell entry pathway of subgroup D Ads.<\/jats:p>","DOI":"10.1128\/jvi.78.8.3897-3905.2004","type":"journal-article","created":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T13:33:15Z","timestamp":1080307995000},"page":"3897-3905","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":146,"title":["Membrane Cofactor Protein Is a Receptor for Adenoviruses Associated with Epidemic Keratoconjunctivitis"],"prefix":"10.1128","volume":"78","author":[{"given":"Eugene","family":"Wu","sequence":"first","affiliation":[{"name":"Departments of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunia A.","family":"Trauger","sequence":"additional","affiliation":[{"name":"Molecular Biology, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Pache","sequence":"additional","affiliation":[{"name":"Departments of Immunology"},{"name":"Fachbereich Biologie, Chemie, Pharmazie, Freie Universita\u0308t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina-Marie","family":"Mullen","sequence":"additional","affiliation":[{"name":"Departments of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Von Seggern","sequence":"additional","affiliation":[{"name":"Departments of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Siuzdak","sequence":"additional","affiliation":[{"name":"Molecular Biology, The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen R.","family":"Nemerow","sequence":"additional","affiliation":[{"name":"Departments of Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.1.42-48.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.8269"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.17.8834-8841.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.4049\/jimmunol.138.11.3850","volume":"138","year":"1987","unstructured":"Ballard, L., T. Seya, J. Teckman, D. M. Lublin, and J. P. Atkinson. 1987. A polymorphism of the complement regulatory protein MCP (membrane cofactor protein or gp45-70). J. Immunol.138:3850-3855.","journal-title":"J. Immunol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3923","DOI":"10.4049\/jimmunol.141.11.3923","volume":"141","year":"1988","unstructured":"Ballard, L. L., N. S. Bora, G. H. Yu, and J. P. Atkinson. 1988. Biochemical characterization of membrane cofactor protein of the complement system. J. Immunol.141:3923-3929.","journal-title":"J. Immunol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1160"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004680200"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5304.1320"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5444.1579"},{"key":"e_1_3_2_11_2","first-page":"3579","volume":"34","year":"1993","unstructured":"Bora, N. S., C. L. Gobleman, J. P. Atkinson, J. S. Pepose, and H. J. Kaplan. 1993. Differential expression of the complement regulatory proteins in the human eye. Investig. Ophthalmol. Vis. Sci.34:3579-3584.","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.11.2911"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.11.5375-5380.2001"},{"key":"e_1_3_2_14_2","first-page":"163","volume":"199","year":"1995","unstructured":"Chroboczek, J., R. W. H. Ruigrok, and S. Cusack. 1995. Adenovirus fiber. Curr. Top. Microbiol. Immunol.199:163-200.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01408.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.8.3661-3673.1990"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80071-L"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036304"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1392-1398.1999"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm952"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1973.03220340013003"},{"key":"e_1_3_2_22_2","first-page":"55","volume":"57","year":"1981","unstructured":"Harnett, G. B., and W. A. Newnham. 1981. Isolation of adenovirus type 19 from the male and female genital tracts. Br. J. Vener. Dis.57:55-57.","journal-title":"Br. J. Vener. Dis."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2509"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10382"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.4.2798-2802.1999"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.25.15148"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90038-D"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4841857.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.15.7210-7214.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01070-3"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"4415","DOI":"10.4049\/jimmunol.156.11.4415","volume":"156","year":"1996","unstructured":"Liszewski, M. K., and J. P. Atkinson. 1996. Membrane cofactor protein (MCP; CD46) isoforms differ in protection against the classical pathway of complement. J. Immunol.156:4415-4421.","journal-title":"J. Immunol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60868-8"},{"key":"e_1_3_2_33_2","first-page":"286","volume":"18","year":"1998","unstructured":"Lukashok, S. A., and M. S. Horwitz. 1998. New perspectives in adenovirus. Curr. Clin. Top. Infect. Dis.18:286-304.","journal-title":"Curr. Clin. Top. Infect. Dis."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.6.2303"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00090-5"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8669-8675.1998"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.10.6025-6032.1993"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1507"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.2.10.1064-1075.1968"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.1.93"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050071"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.3109\/02713689209001785"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1654(1998100)8:4<187::AID-RMV221>3.0.CO;2-S"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5444.1568"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.7909-7915.1998"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81678-5"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.17.9183-9191.2003"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.22.10274-10286.2000"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.5.2850-2857.1995"},{"key":"e_1_3_2_50_2","first-page":"2728","volume":"33","year":"2003","unstructured":"Swenson, P. D., M. S. Lowens, C. L. Celum, and J. C. Hierholzer. 2003. Adenovirus types 2, 8, and 37 associated with genital infections in patients attending a sexually transmitted disease clinic. J. Clin. Microbiol.33:2728-2731.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.4.1839"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90231-E"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0703"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.13.7225-7235.2003"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(94)00126-X"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.8.3897-3905.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.8.3897-3905.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T01:40:23Z","timestamp":1682646023000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.78.8.3897-3905.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,15]]},"references-count":54,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,4,15]]}},"alternative-id":["10.1128\/JVI.78.8.3897-3905.2004"],"URL":"https:\/\/doi.org\/10.1128\/jvi.78.8.3897-3905.2004","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1020214.231430","asserted-by":"object"}]},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,15]]},"assertion":[{"value":"2003-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-12-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}