{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:36:34Z","timestamp":1761154594331},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2004,4,15]],"date-time":"2004-04-15T00:00:00Z","timestamp":1081987200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2004,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Adaptive immune responses in which CD8<jats:sup>+<\/jats:sup>T cells recognize pathogen-derived peptides in the context of major histocompatibility complex class I molecules play a major role in the host defense against infection with intracellular pathogens. Cells infected with intracellular bacteria such as<jats:italic>Listeria monocytogenes<\/jats:italic>,<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium, or<jats:italic>Mycobacterium tuberculosis<\/jats:italic>are directly lysed by cytotoxic CD8<jats:sup>+<\/jats:sup>T cells. For this reason, current vaccines for intracellular pathogens, such as subunit vaccines or viable bacterial vaccines, aim to generate robust cytotoxic T-cell responses. In order to investigate the capacity of a herpes simplex virus type 1 (HSV-1) vector to induce strong cytotoxic effector cell responses and protection from infection with intracellular pathogens, we developed a replication-deficient, recombinant HSV-1 (rHSV-1) vaccine. We demonstrate in side-by-side comparison with DNA vaccination that rHSV-1 vaccination induces very strong CD8<jats:sup>+<\/jats:sup>effector T-cell responses. While both vaccines provided protection from infection with<jats:italic>L. monocytogenes<\/jats:italic>at low, but lethal doses, only rHSV-1 vaccines could protect from higher infectious doses; HSV-1 induced potent memory cytotoxic T lymphocytes that, upon challenge by pathogens, efficiently protected the animals. Despite the stimulation of relatively low humoral and CD4-T-cell responses, rHSV-1 vectors are strong candidates for future vaccine strategies that confer efficient protection from subsequent infection with intracellular bacteria.<\/jats:p>","DOI":"10.1128\/jvi.78.8.4020-4028.2004","type":"journal-article","created":{"date-parts":[[2004,3,26]],"date-time":"2004-03-26T18:33:15Z","timestamp":1080325995000},"page":"4020-4028","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Protection from Bacterial Infection by a Single Vaccination with Replication-Deficient Mutant Herpes Simplex Virus Type 1"],"prefix":"10.1128","volume":"78","author":[{"given":"Henning","family":"Lauterbach","sequence":"first","affiliation":[{"name":"Institute for Immunology, Ludwig-Maximilians-University, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen M.","family":"Kerksiek","sequence":"additional","affiliation":[{"name":"Institute for Immunology, Ludwig-Maximilians-University, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk H.","family":"Busch","sequence":"additional","affiliation":[{"name":"Technische Universita\u0308t Munich, Institute for Medical Microbiology and Hygiene, 81675 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Berto","sequence":"additional","affiliation":[{"name":"University of Ferrara, Department of Experimental and Diagnostic Medicine, 44100 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Bozac","sequence":"additional","affiliation":[{"name":"University of Ferrara, Department of Experimental and Diagnostic Medicine, 44100 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penelope","family":"Mavromara","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Manservigi","sequence":"additional","affiliation":[{"name":"University of Ferrara, Department of Experimental and Diagnostic Medicine, 44100 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto L.","family":"Epstein","sequence":"additional","affiliation":[{"name":"University Claude-Bernard Lyon 1, Centre de Genetique Moleculaire et Cellulaire, C.N.R.S., UMR 5534, 69622 Villeurbanne Cedex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peggy","family":"Marconi","sequence":"additional","affiliation":[{"name":"University of Ferrara, Department of Experimental and Diagnostic Medicine, 44100 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Brocker","sequence":"additional","affiliation":[{"name":"Institute for Immunology, Ludwig-Maximilians-University, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.9.4968"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1685-1693.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.1.16"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.8.3678-3687.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80540-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210391497"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.2.834"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70296-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.4049\/jimmunol.163.1.322","volume":"163","year":"1999","unstructured":"Cornell, K. A., H. G. Bouwer, D. J. Hinrichs, and R. A. Barry. 1999. Genetic immunization of mice against Listeria monocytogenes using plasmid DNA encoding listeriolysin O. J. Immunol.163:322-329.","journal-title":"J. Immunol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.12.6994"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.12.7102"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4174-4176.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199710163371601"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/335259a0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(99)80016-8"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/35095558"},{"key":"e_1_3_2_18_2","unstructured":"Kaufmann S. H. E. 1998. Immunity to intracellular bacteria p. 1335-1371. In W. E. Paul (ed.) Fundamental immunology. Raven Press Ltd. New York N.Y."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90084-1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300497"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300755"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300766"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8926"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9716"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.4049\/jimmunol.153.7.3116","volume":"153","year":"1994","unstructured":"Ladel, C. H., I. E. Flesch, J. Arnoldi, and S. H. Kaufmann. 1994. Studies with MHC-deficient knock-out mice reveal impact of both MHC I- and MHC II-dependent T cell responses on Listeria monocytogenes infection. J. Immunol.153:3116-3122.","journal-title":"J. Immunol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1064571"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.5.1100"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00259-4"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.681-686.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.2.589"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0328"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.17.7745-7754.2000"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.138.2.342"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.9293"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.5.1273"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3402"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119149"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.8.4204"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5386.121"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5319.1684"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3041-3047.2001"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0896-888"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910143411602"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.4998"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90215-1"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.8.4020-4028.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.78.8.4020-4028.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T05:40:30Z","timestamp":1682660430000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.78.8.4020-4028.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,15]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,4,15]]}},"alternative-id":["10.1128\/JVI.78.8.4020-4028.2004"],"URL":"https:\/\/doi.org\/10.1128\/jvi.78.8.4020-4028.2004","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,15]]},"assertion":[{"value":"2003-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-11-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}