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We detected the disease-specific, protease-resistant prion protein (PrP<jats:sup>bse<\/jats:sup>) in splenic DC purified by magnetic cell sorting 45 days after intraperitoneal inoculation of BSE prions in immunocompetent mice. We showed that bone marrow-derived DC (BMDC) from wild-type or PrP-null mice acquired both PrP<jats:sup>bse<\/jats:sup>and prion infectivity within 2 h of in vitro culture with a BSE inoculum. BMDC cleared PrP<jats:sup>bse<\/jats:sup>within 2 to 3 days of culture, while BMDC infectivity was only 10-fold diminished between days 1 and 6 of culture, suggesting that the infectious unit in BMDC is not removed at the same rate as PrP<jats:sup>bse<\/jats:sup>is removed from these cells. Bone marrow-derived plasmacytoid DC and bone marrow-derived macrophages (BMM) also acquired and degraded PrP<jats:sup>bse<\/jats:sup>when incubated with a BSE inoculum, with kinetics very similar to those of BMDC. PrP<jats:sup>bse<\/jats:sup>capture is probably specific to antigen-presenting cells since no uptake of PrP<jats:sup>bse<\/jats:sup>was observed when splenic B or T lymphocytes were incubated with a BSE inoculum in vitro. Lipopolysaccharide activation of BMDC or BMM prior to BSE infection resulted in an accelerated breakdown of PrP<jats:sup>bse<\/jats:sup>. Injected by the intraperitoneal route, BMDC were not infectious for alymphoid recombination-activated gene 2<jats:sup>0<\/jats:sup>\/common cytokine \u03b3 chain-deficient mice, suggesting that these cells are not capable of directly propagating BSE infectivity to nerve endings.<\/jats:p>","DOI":"10.1128\/jvi.80.10.4656-4663.2006","type":"journal-article","created":{"date-parts":[[2006,4,26]],"date-time":"2006-04-26T22:00:29Z","timestamp":1146088829000},"page":"4656-4663","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Processing of the Bovine Spongiform Encephalopathy-Specific Prion Protein by Dendritic Cells"],"prefix":"10.1128","volume":"80","author":[{"given":"Catherine","family":"Rybner-Barnier","sequence":"first","affiliation":[{"name":"Neurovirologie et Re\u0301ge\u0301ne\u0301ration du Syste\u0300me Nerveux, Departement des Neurosciences"},{"name":"Repliement et Mode\u0301lisation des Prote\u0301ines, Departement de Biologie Structurale et Chimie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Jacquemot","sequence":"additional","affiliation":[{"name":"Neurovirologie et Re\u0301ge\u0301ne\u0301ration du Syste\u0300me Nerveux, Departement des Neurosciences"},{"name":"Repliement et Mode\u0301lisation des Prote\u0301ines, Departement de Biologie Structurale et Chimie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301line","family":"Cuche","sequence":"additional","affiliation":[{"name":"Repliement et Mode\u0301lisation des Prote\u0301ines, Departement de Biologie Structurale et Chimie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gre\u0301gory","family":"Dore\u0301","sequence":"additional","affiliation":[{"name":"Repliement et Mode\u0301lisation des Prote\u0301ines, Departement de Biologie Structurale et Chimie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laleh","family":"Majlessi","sequence":"additional","affiliation":[{"name":"Biologie des Re\u0301gulations Immunitaires, INSERM E352, Departement d'Immunologie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Madeleine","family":"Gabellec","sequence":"additional","affiliation":[{"name":"Perception et Me\u0301moire Olfactive, Departement des Neurosciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Moris","sequence":"additional","affiliation":[{"name":"Virus et Immunite\u0301"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Virus et Immunite\u0301"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Di Santo","sequence":"additional","affiliation":[{"name":"Cytokines et De\u0301veloppement Lymphoi\u0308de"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Cumano","sequence":"additional","affiliation":[{"name":"De\u0301veloppement des Lymphocytes, INSERM U668, Departement d'Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Biologie des Re\u0301gulations Immunitaires, INSERM E352, Departement d'Immunologie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Lazarini","sequence":"additional","affiliation":[{"name":"Neurovirologie et Re\u0301ge\u0301ne\u0301ration du Syste\u0300me Nerveux, Departement des Neurosciences"},{"name":"Repliement et Mode\u0301lisation des Prote\u0301ines, Departement de Biologie Structurale et Chimie"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1437"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205001009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1189\/jlb.72.6.1075","volume":"72","year":"2002","unstructured":"Aucouturier, P., and C. 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