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In contrast to the effects on bone marrow- and monocyte-derived DCs, the current study shows that Ad infection of murine BALB\/c lung DCs in vitro and in vivo suppresses DC-induced T-cell proliferation. The effect of Ad on DCs was not due to a downregulation of major histocompatibility complex or costimulatory molecules. Analysis of the production of interleukin-12 (IL-12), alpha interferon (IFN-\u03b1), and IFN-\u03b3 by the Ad-infected DCs shows no significant differences over noninfected control lung DCs. Ad-induced suppression was not due to a deficiency of IL-2 or other DC-secreted factors and was dependent on viral protein synthesis, as UV irradiation of Ad abrogated the suppressive effect. Results suggest that Ad-infected DCs induce T cells to be nonresponsive to IL-2 during primary coculture, as the addition of IL-2 in secondary cultures recovered T-cell proliferation. In vivo studies supported in vitro results showing that Ad infection resulted in lung T cells with decreased proliferative ability. This study demonstrates that Ad infection induces local immunoincompetence by altering DC-T-cell interactions.<\/jats:p>","DOI":"10.1128\/jvi.80.4.1826-1836.2006","type":"journal-article","created":{"date-parts":[[2006,1,26]],"date-time":"2006-01-26T18:02:43Z","timestamp":1138298563000},"page":"1826-1836","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Pulmonary Immunity to Viral Infection: Adenovirus Infection of Lung Dendritic Cells Renders T Cells Nonresponsive to Interleukin-2"],"prefix":"10.1128","volume":"80","author":[{"given":"Allison T.","family":"Thiele","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina L.","family":"Sumpter","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna A.","family":"Walker","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Xu","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheong-Hee","family":"Chang","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Bacallao","sequence":"additional","affiliation":[{"name":"Nephrology"},{"name":"Indiana Center for Biologic Microscopy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh","family":"Kher","sequence":"additional","affiliation":[{"name":"Nephrology"},{"name":"Indiana Center for Biologic Microscopy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Wilkes","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Immunology"},{"name":"Medicine"},{"name":"Center for Immunobiology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.5.2454-2459.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"3960","DOI":"10.4049\/jimmunol.138.11.3960","volume":"138","year":"1987","unstructured":"Andersson, M., A. 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